Blood Typing and Blood Stain Patterns Quick Review
Blood Typing and Blood Stain Patterns
Quick Review • Where antigens found? • Where antibodies found?
Blood Typing • Serology describes the lab tests that use specific antigen and serum antibody reactions • To determine the A-B-O blood type, two antiserums are needed, anti-A and anti-B • Patient blood cells are mixed with these antiserums for agglutination or the absence of agglutination to determine blood type
Results • Type A is agglutinated by anti -A serum • Type B is agglutinated by anti -B serum • Type AB is agglutinated by both • Type O is agglutinated by neither • Why does it work this way? A B
Bloodstain Patterns • The location, distribution, and appearance of bloodstains and spatters are useful for interpreting and reconstructing events that produced the bleeding • They need to trace the direction, dropping distance, and angle of impact of a stain • They include:
Surface Texture • The harder and less porous the surface, the less spatter • If it hits glass, which is nonporous, the stain should be circular without much splatter • If it hits a cotton sheet, which is porous, more splatter will be seen
Direction of Travel • Interpretation of the direction can be seen by the stain’s shape • The pointed end of a bloodstain always faces its direction of travel before striking the surface
Impact Angles • Its possible to determine the impact angle of blood on a flat surface by measuring the degree of circular distortion of the stain. • If blood strikes a surface at 90 o, it produces a nearly circular stain. • As the angle decreases, the stain gets longer. • To determine the angle, use the formula Sin ө=width/length.
Trigonometry: Calculating impact angles
Recreating Angles of Impact • Drop patterns are created by droplets landing at different angles from the same height. • In order to recreate a blood stain at different angles, place a piece of paper on the clip board and align the clipboard with the required angle line using a protractor. Hold a bottle of blood at a desired height from the top of a table. • GENTLY squeeze the bottle so that ONE drop of blood is released and lands on the paper. • Continue testing by dropping blood from at the same height being used at each of the other angles. Compare the angles. Clipboard & Paper Height of 50 cm Angle Guide
Origin of Blood Spatter • The origin can be established by drawing straight lines through the long axis of several individual blood stains • By examining at least two drops of blood, you will be able to determine where the injured person was located when the injury occurred
What does the abbreviation BPA represent? Bloodstain Pattern Analysis What can an investigator learn from the analysis of a blood spatter? Type and velocity of weapon Number of blows Handedness of assailant (right or left-handed) Position and movements of the victim and assailant during and after the attack Which wounds were inflicted first Type of injuries How long ago the crime was committed Whether death was immediate or delayed Source: http: //science. howstuffworks. com/bloodstain-pattern-analysis 1. htm http: //www. crimescenetwo. com/img/popup/book 2 p 2. jpg
How is blood evidence detected at a crime scene? Light Source They may use a high-intensity light or UV lights to help them find traces of blood as well as other bodily fluids that are not visible under normal lighting conditions. Blood Reagent Tests These tests, referred to as presumptive tests, are used to detect blood at crime scenes based upon the properties of hemoglobin in the blood. Examples: Phenolphthalein is a chemical that is still utilized today and is usually referred to as the Kastle-Meyer test and produces a pink color when it reacts with hemoglobin. Kastle-Meyer Hema. Stix is a strip that has been coated with tetramethylbenzidine (TMB) and will produce a green or bluegreen color with the presence of hemoglobin. Hema. Stix
Luminol This chemical is used by crime scene investigators to locate traces of blood, even if it has been cleaned or removed. Investigators spray a luminol solution throughout the area under investigation and look for reactions with the iron present in blood, which causes a blue luminescence. One problem is that other substances also react, such as some metals, paints, cleaning products, and plant materials. Another problem is that the chemical reaction can destroy other evidence in the crime scene. Fluorescein This chemical is also capable of detecting latent or old blood, similar to luminol. It is ideal for fine stains or smears found throughout a crime scene. After the solution has been sprayed onto the substance or area suspected to contain blood, a UV light and goggles are used to detect any illuminated areas, which appear greenish-white if blood is present. It may also react to many of the same things as luminol (copper and bleach). Luminol Reaction Fluorescein Reaction in UV Light LCV or Leuco Crystal Violet, is one type of chemical process that is used for blood enhancement. Using this test helps to make the blood evidence more visible so it can be photographed analyzed. LCV
Bloodstain Pattern Analysis Terms • Spatter – Bloodstains created from the application of force to the area where the blood originated. • Origin/Source – The place from where the blood spatter came from or originated. • Angle of Impact – The angle at which a blood droplet strikes a surface. • Parent Drop – The droplet from which a satellite spatter originates. • Satellite Spatters – Small drops of blood that break of from the parent spatter when the blood droplet hits a surface. • Spines – The pointed edges of a stain that radiate out from the spatter; can help determine the direction from which the blood traveled. Satellite Spatters Spines Parent Drop
Types of Bloodstain Patterns • Passive Bloodstains – Patterns created from the force of gravity – Drop, series of drops, flow patterns, blood pools, etc. • Projected Bloodstains – Patterns that occur when a force is applied to the source of the blood – Includes low (5 ft/s or less), medium (5 -25 ft/s), or high (greater than 25 ft/s and gives a fine mist appearance) impact spatters, cast-off blood that is thrown from an object in motion, arterial spurting when blood exits the body under pressure, expiratory blood blown out of the nose, mouth, or wound. • Transfer or Contact Bloodstains – These patterns are created when a wet, bloody object comes in contact with another surface; may be used to identify an object or body part. – A wipe pattern is created from an object moving through a bloodstain, a swipe pattern is created from an object leaving a bloodstain, .
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