Blackworm Toxin Ibuprofen Introduction Ibuprofen is a NSAID
Blackworm Toxin: Ibuprofen Introduction: Ibuprofen is a NSAID, which means that it is a Non. Steroidal Anti-Inflammatory Drug. NSAIDs reduce hormones that cause inflammation and blocks production of prostaglandin. Prostaglandins are substances our body releases in response to pain. Ibuprofen’s chemical formula is C 13 H 18 O 2. Ibuprofen is the toxin we are testing on our blackworms. The Blackworms have transparent skin so their pulse rate can be easily recorded. Hypothesis: If we add Ibuprofen to the water, that the blackworm is in, then the pulse rate of the blackworm will be lower than the pulse rate of the blackworm in spring water Dependent Variable: Pulse rate of blackworms Independent Variable: 1, 200 mg tablet of Ibuprofen Constants: Type of worm, Type of spring water, temperature, microscope, time exposure to ibuprofen Materials: -Ibuprofen -Blackworms -Microscope -10 containers -Well Slides -Recovery dish -Stopwatch -Spring water -Ibuprofen solution Chemical Structure of Ibuprofen: Albion Central School, GCC Bio 100 Meghan Hurley and Alyce Miller Pulse rate in low, medium, and high concentrations of Ibuprofen Objectives: -Research the effects of Ibuprofen on the pulse rate of blackworms -Demonstrate the ability to design a neat and organized experiment -Count pulse rates -Define relationship between dependent and independent variable Observations: We dissolved and warmed tablet but it did not fully dissolve. There was undissolved substance that we believe was the coating. It was pink. Worm died at 100 m. L of water. Procedure: 1) Crush one 200 mg Ibuprofen tablet and dissolve in 200 m. L of spring water. 2) Put the worm into container with 10 m. L of Ibuprofen solution. (None, low, medium, high concentrations) 3) Leave worm in solution for 30 seconds. 4) Record pulse rate of worm for one minute. 5) Put worm in recovery dish. 6) Repeat 10 times (steps 2 -5). 7) Repeat steps 2 -6 in different solutions. (none, low, medium, high concentrations) Conclusion: The data we collected did not support our hypothesis. We thought the pulse rate would decrease, but it in fact did the exact opposite. The mean in spring water was 13. 5 and the mean in high Ibuprofen solution was 18. 9. The problems we faced consisted of us not being able to filter and dissolve the ibuprofen easily. So because of this we had to warm up the solution and when it still didn't filter, we had to use filter paper. We did not expect the heart rate to be higher in ibuprofen solution because after researching, it says that ibuprofen stops blood flow. What is the coating made out of? It does not dissolve in water. References: http: //www. rxlist. com/ibuprofen-drug. htm http: //www. medicalnewstoday. com/articles/161071. php
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