Biotechnology Tools SEROLOGICAL PIPETS Blue Pump 2 m

Biotechnology Tools

SEROLOGICAL PIPETS Blue Pump 2 m. L, Green Pump 10 m. L , Red Pump 25 m. L Never mouth pipet Solutions should never touch the cotton plug Pipetting should always be done at eye level Pipet always be in a vertical position Numbers represent how much you have pipetted Example if I fill the pipet to the 0 line and pipet 1 m. L the meniscus will touch the 1 m. L line

SEROLOGICAL PIPETS Without Touching the pipet tip, remove it from the plastics sleeve Insert the wide end into the pipet pump Twist the pipet gently into the pump to get a tight seal Fill the pipet a bit above the amount desired and then slowly lower the meniscus to that line. Remove the pipet and discard it into an appropriate discard container. Pipet can be reused for same solution only!!!!

Serological Pipet Basics

LAB 3 A MEASURING SMALL VOLUMES Solutions add across the data tables You must uses the different color dyes to get the final solution You will compare your final solution to mine and it should be the same volume and roughly the same color. I. Red II. Blue III. Green IV. Yellow V. Purple

Using a Micropipette

Choosing and Setting a Micropipette 1. 2. Choose the smallest pipet that can measure your volume Set the pipet n n Numbers get larger when you move the dial to the right Numbers get smaller when you move the dial to the left

Setting Micropipet Practice For each of the following-Draw the correct setting!!! A. B. C. D. E. P 20 set to 15. 85 P 100 set to 56. 5 P 1000 set to 265 P 200 set to 19. 5 P 20 set to 3. 5

USING A CENTRIFUGE TAB Put the tubes opposite each other in the centrifuge. Tubes should be loaded with tab facing the inside Make a counterbalance for the centrifuge tube you want to put in the centrifuge. The masses, not volumes, of the tubes should be as close as possible! Unbalanced tubes may permanently damage the centrifuge If balanced it will be almost silent

USING A CENTRIFUGE Close the lid, will turn on if balanced Do not open the lid until it stops spinning completely Remove the tubes carefully after the centrifuge so they don’t get mixed up again Most often used to separate suspensions in liquids. Also used to mix small amounts of liquid and relocate to bottom of tube.

Using a Centrifuge

BACKGROUND ON SPECTROPHOTOMETER

A spectrophotometer is a device used to measure light at a specific wavelength. It consists of two parts: a spectrometer and a photometer. The spectrometer provides light at a specific wavelength. The photometer measures how intense the light is. By calculating the amount of light that a solution is able to absorb and applying Beer's Law, the spectrophotometer can determine the concentration of a colored solution.

SPECTROPHOTOMETER VIDEO https: //www. youtube. com/watch? v=LL 36 z. KV 12 zs