Biosensor Technology An Innovative Option for the Food
Biosensor Technology An Innovative Option for the Food Sector Dr H Schnerr Leatherhead Food International Ltd. , Randalls Road, Leatherhead, Surrey, KT 22 7 RY +44 (0) 1372 822203 hschnerr@leatherheadfood. com www. leatherheadfood. com
SPR Suppliers Company Instrument Website Biacore http: //www. biacore. com Affinity Sensors IAsys http: //www. affinity-sensors. com IBIS Technologies IBIS http: //www. ibis-spr. nl Texas Instruments SPREETA http: //www. ti. com BIOSUPLAR http: //www. biosuplar. de GWC technologies SPRimager http: //www. gwctechnologies. com Reichert Refractometer. SR 7000 http: //www. reichertai. com Analytical µ-Systems INIX http: //www. inex. org. uk
Objectives of the Experiment Kinetic Rate Analysis: How FAST? ka, kd KD = kd/ka, KA = ka/kd Affinity Analysis: How STRONG? KD, KA Relative Ranking Concentration Analysis: How MUCH? Active Concentration Solution Equilibrium Inhibition Yes/No Data Ligand Fishing
SPR technology • Label-free • Real-time • Unique, high quality data on molecular interactions • Simple assay design • Robust and reproducible • Walk-away automation • Minimal sample preparation • Rapid method
Biomolecular Binding in Real Time Binding Event Real-time Sensorgram
Biomolecular Binding in Real Time Principle of Detection LED Reflected light Polarised light CCD Reflected light intensity a 1 YYYY Flow cell a 1 RU Angle of reflection a 2 a 1 Reflected light intensity Time a 2 YYYY Sensor chip a 2 Angle of reflection
Flexibility in Assay Design Direct measurement Indirect measurement Direct Binding Assay (DBA) Surface competition assay (SCA) Inhibition in solution assay (ISA)
Assays Relevant to Industry Food Quality Vitamins Biotin Folic Acid B 12 Pantophenic Acid (B 5) Authenticity Nonmilk Protein in Milk Powder GMO Testing Process-dependent Food Change Pasteurisation of Milk
Assays Relevant to Industry Food Safety Mycotoxins DON Aflatoxin Antibiotics, growth promoters and veterinary drug residues Streptomycin Chloramphenicol -agonists Food Pathogens Salmonella E. coli O 157: H 7
Assays Relevant to Industry Food Related Studies Protein-Polysaccharide-Interactions -casein and -/ -carrageenan Binding Interaction Studies of Food Pathogens Salmonella and Collagen/Muscle Proteins E. coli O 157: H 7 and Collagen Interactions with membranes, receptors etc. Looking for Bioactive Appetite Suppressants
Assays Relevant to Industry Life Science/Drug Discovery Proteomics Ligand Fishing SPR/MS Antibody Characterisation Isotyping Affinity Kinethic Target ID Elucidate Disease Mechanisms Define Drugs or Diagnostic Markers
LFI and Biosensors • Sensor Development - Biotin/Folic Acid • Pathogen Detection - Salmonella • Allergen Testing • Interaction studies - Polysaccharide - Protein • Method Evaluation & Validation • Routine Analysis - Qflex®Kits for Vitamins
Summary • SPR detects binding events as changes in mass at the chip surface • Real-time kinetic measurements • Label-free technology • Qualitative rankings • Flexibility in assay design • Minimal sample preparation • One platform for many different assays
Biosensor Technology An Innovative Option for the Food Sector
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