BIOPROCESS LAB B 2010 KAREN LANCOUR BioProcess Lab

BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR karenlancour@charter. net

Bio-Process Lab (B) n n n Event Description - lab-oriented competition involving the fundamental science processes of a middle school biology lab program Event – lab practical in stations Event Parameters – be sure to check the rules for resources allowed, type of goggles needed.

TRAINING MATERIALS n n Training Handout – content Training Handout Event Supervisor Guide – sample Event Supervisor Guide stations, process skills, methods Internet Resource – links to on-line Internet Resource courses, lab manuals, notes, sites Sample Tournaments – sample stations Sample Tournaments with key

Basic Science Process Skills Observing n Measuring n Inferring n Classifying n Predicting n Communicating n

Integrated Science Process Skills n n n n n Formulating Hypothesis Identifying Variables Defining Variables Operationally Describing Relationships Between Variables Designing Investigations Experimenting Acquiring Data Analyzing Investigations and Their Data Understanding Cause and Effect Relationships Formulating Models

Student Preparation n n n Team work skills Time limits Answering questions Measurement and Calculations Reference materials Practice using labs and lab manuels Construct sample stations

Compound Microscope § § § § Parts Making wet mount Appearance of objects Movement of objects Magnification Changing objects Estimating size of objects Field diameter & area

Principles of Microscopy

Measuring objects

Stereomicroscope n n n Parts Appearance of objects Magnification Advantages Uses Observing objects

Electronic Balance Capacity n Units n Tare or Zero n Err n n n Using the Balance Advantages & Disadvantages

Triple Beam Balance n n n Capacity – auxillary weights Units – numbered and unnumbered increments Tare Using the Balance Advantages & Disadvantages

Measuring Liquids n Meniscus – read bottom Capacity and Range Graduations – numbered and unnumbered increments Readability Making measurements n Estimating n n

Metric ruler and calipers n n n n Capacity and Range Numbered and unnumbered increments cm vs mm Uses of each Making measurements Estimating Vernier Scale

Thermometers n n n Capacity and Range Do not start at zero Numbered and unnumbered increments Uses of each Making measurements Estimating

Probes n n n n Graphing calculator Easy link or CBL Probe Collect data onto calculator Transfer data to computer Graph analysis Quick data collection

Data Presentation and Analysis- Data Tables n n n Format Title Units of measurement Numbering Tables Source Leg (thigh) Time of 40 yard Length (cm) dash (sec) 24 9 31 9. 2 37 11 38 10 39 8. 2 42 8. 4 55 9. 3 62 9

Data Presentation and Analysis. Graphs n n n n Graph – types X vs Y axis Scaling axis Plotting points Human Error Curve or best fit line Labeling

Human Mistakes vs Experimental Errors Human Mistakes – carelessness n Experimental Error – instrument variation or technique n Random Error – chance variation n Systematic Error – system used for designing or conducting experiment n

A Sample Dichotomous Key n 1. wings covered by an exoskeleton. . . go to step 2 1. wings not covered by an exoskeleton. . go to step 3 n 2 body has a round shape. . . . . ladybug 2 body has an elongated shape. . . . grasshopper n 3. wings point out from the side of the body. . dragonfly 3 wings point to the posterior of the body. . . Housefly n Note: There should be one less step than the total number of organisms to be identified in your dichotomous key.

Sample Stations – Population Density n n n Measure sample area Determine population density for symbols Assign an organism to symbols Form a food chain Evaluate sample – predict techniques, etc.

Sample Station – Dichotomous Key n n Have specimens of leaves Formulate a key or use a key to identify specimens

Sample Station - Measurement n n Identify range, capacity, incrementation Do measurement – estimate last digit

Sample Station – Experiment Analysis n n n Analyze the design of the experiment Identify variables Explain results – form conclusions

Sample Station – Microscopy n Determine the diameter of the field and the length of one cell in mm and convert to mcm.

Sample Station – Data Analysis n n n Making hypotheses Food web analysis Eating habit analysis Predictions and conclusions Inferences

Sample Station – p. H n Determine the p. H of various solutions using either p. H probe or p. H test papers.