Biological Nanomachine Architecture and Designed Function with SAXS

Biological Nanomachine Architecture and Designed Function with SAXS through DOE/BER: IDAT Emergent DOE/BER methodologies for using SAXS to inform on protein design “Designing and defining dynamic protein cage nanoassemblies in solution” Science Advances Dec 14 th 2016 IDAT: Greg L. Hura & John A. Tainer collaboration with UCLA-DOE Institute: Yen Ting Lai and Todd O. Yeates

Structure and Mechanisms of Complex Biological Pathways with Combined X-ray Methods through DOE/BER: IDAT Biology requires complex protein and DNA assemblies that can only be characterized by multiple techniques at multiple scales Scaling Atomic to Cellular Distances for Microbial Epigenetic Control An Intrinsically Disordered APLF Links Ku, DNAPKcs and XRCC 4 -DNA Ligase IV in an Extended Flexible Non-Homologous End Joining Complex. JBC By: Hammel M, Tainer JA, et al NOV 14 2016 Molecular architecture of the human sperm IZUMO 1 and egg JUNO fertilization complex NATURE By: Aydin, Halil; et al. JUN 23 2016 Polycomb repressive complex 2 structure with inhibitor reveals a mechanism of activation and drug resistance NATURE COMMUNICATIONS Industrial Collaborators: Pfizer FEB 10 2016 Cellular X-ray Tomography Mutant 1 Hammel M, Larabell C. Tainer JA et al Science Advances July 2016 Mutant 2