Bioinnovation at your service Company presentation and ProductsServices

Bioinnovation at your service Company presentation and Products/Services Portfolio 2016

About us Biomedal S. L. is a biotechnology company whose mission is to develop and commercialize new technologies, services and products for research, bio and food industries and diagnostics. • Experience of more for than 15 years in research and product development for food safety • Sales: customers and distributors in more than 50 countries. • Staff: 29 (Ph. Ds, lab technicians, salespeople and marketing) • Company turnover increasing every year. • Headquarter and two laboratories in Sevilla, Spain. Headquarters Biomedal Diagnostics Laboratory Biomedal Life Sciences Laboratory

Biomedal Diagnostics Focused on food safety: Gluten. Tox® innovative rapid gluten detection kits; Aler. Tox® rapid allergen detection kits; Oleotest® rapid test for the quality control of frying oil; 5 -in-1 Water Test; and a full range of food analysis services such as gluten quantification analysis service under ISO 17025 accreditation and other food allergens, pathogens and contaminants detection services. i. VYDAL Focused on in-vitro-diagnostics (IVD) products and solutions for celiac disease diagnosis, gluten free diet monitoring (i. VYLISA GIP-S) and other auto-immune disease markers for posttransplant rejection detection (anti human-GSTT 1). Biomedal Life Science Focused on tools for research and bioprocessing industries: CASCADE™ protein expression system; innovative protein purification technologies such as HISTAG Two Phase and LYTAG Two Phase purification systems; Pro. Pure®, a line of protein A and protein G affinity resins; and a large variety of cloning vectors and strains.

Biomedal Diagnostics: Food Safety products Gluten. Tox products offer practical tools to detect the most immunogenic fraction of gluten from wheat, rye, barley and some varieties of oat. Oleotest is a rapid test to report the quality of used frying oil by estimation of the polar compound concentration. Rapid kits for food proteins residues detection on foodstuff and surfaces. Easily detects residues from: milk (casein and Blactoglobulin), egg, crustacean, fish, soy, peanut, hazelnut and almond.

Food Safety products: Gluten detection

Advantages ü Gluten. Tox Home gives you the option to test food at a threshold of 20 ppm or 5 ppm (maximal sensitivity of the test). ü Results in less than 20 minutes. ü No special equipment needed. ü Easy storage at room temperature (35ºF— 85ºF). Materials

Advantages ü Detects gluten from wheat, barley, rye and oat To avoid accidental gluten contamination ü Adjustable sensitivity: 5, 10, 20, 40 ppm of gluten ü for restaurant kitchens, cafeterias, catering, manufacturers, etc. ü Long-lasting and shelf-stable ü from incoming ingredients ü Results in less than 20 minutes ü within shared facilities ü No special equipment required ü via cooking surfaces and countertops ü Unique method to detect oat toxicity ü in final products ü Safe and easy to use

Advantages Industrial application ü Detection limit 3 ppm (<20 ppm is considered “gluten free”) ü Semi-quantification of gluten in food ü Results in 1 hour food samples and 15 minutes for working surfaces ü Trace gluten contamination in food and working surfaces ü Suitable for most hydrolysed food ü Monitoring of gluten detoxification ü Specific to the toxic fraction of gluten from wheat, barley, rye and oat ü Implementation and fulfillment of HACCP, ISO 22000, BRC, and IFS compliance ü No cross-reactivity with soy, rice or corn ü Unique method to detect oat toxicity ü Gluten-free food quality control

Gluten. Tox Reader A rapid, quantitative and reliable way to scan your Gluten. Tox® Sticks Plus ü Objectives results ü Quantitatives results ü Highly-Sensible method ü Exact concentration values obtained ü Storage capacity of 100 scans ü Extremely easy to use ü Only one working dilution required How to use The perfect device for the detection and quantification of Gluten during the gluten free processing in food industries Insert the Gluen. Tox® Sticks Plus test strip containing the diluted sample Press the ‘Start’ Button Get your quantitative result in PPM in less than one minute

$Gluten. Tox ELISA G 12 ü Specific to the immunotoxic fraction of gluten from$

Gluten. Tox ELISA G 12 ü Specific to the immunotoxic fraction of gluten from wheat, rye, barley and oat. ü No cross reactivity with any safe food for celiacs, including soy, rice and corn. ü Unique method to detect potential toxic oats. ü Gluten free food quality control of final products. ü Monitoring of gluten detoxification. ü Implementation and compliance of HACCP, ISO 22000, BRC, and IFS conformity. ü Limit of detection: 3 ppm ü Limit of detection: 0, 6 ppm ü Results in 1, 5 hours ü Results in 2, 5 hours ü Valid for hydrolyzed food testing ü Valid for a wide range of food matrices

Scientific publications Identification and in vitro reactivity of celiac immunoactive peptides in an apparent gluten-free beer. Real A, Comino I, Moreno Mde L, López-Casado MÁ, Lorite P, Torres MI, Cebolla Á, Sousa C. PLo. S One. 2014 Jun 25; 9(6): e 100917. Select item 242783592. Detection of specific Ig. A antibodies against a novel deamidated 8 -Mer gliadin peptide in blood plasma samples from celiac patients. Vallejo-Diez S, Bernardo D, Moreno Mde L, Muñoz-Suano A, Fernández-Salazar L, Calvo C, Sousa. C, Garrote JA, Cebolla A, Arranz E. PLo. S One. 2013 Nov 22; 8(11): e 80982. Molecular and immunological characterization of gluten proteins isolated from oat cultivars that differ in toxicity for celiac disease. Real A, Comino I, de Lorenzo L, Merchán F, Gil-Humanes J, Giménez MJ, López-Casado MÁ, Torres MI, Cebolla Á, Sousa C, Barro F, Pistón F. PLo. S One. 2012; 7(12): e 48365. Significant differences in coeliac immunotoxicity of barley varieties. Comino I, Real A, Gil-Humanes J, Pistón F, de Lorenzo L, Moreno Mde L, López-Casado MÁ, Lorite P, Cebolla A, Torres MI, Barro F, Sousa C. Mol Nutr Food Res. 2012 Nov; 56(11): 1697 -707. doi: 10. 1002/mnfr. 201200358. Epub 2012 Sep 12. Immunological determination of gliadin 33 -mer equivalent peptides in beers as a specific and practical analytical method to assess safety for celiac patients. Comino I, Real A, Moreno Mde L, Montes R, Cebolla A, Sousa C. J Sci Food Agric. 2013 Mar 15; 93(4): 933 -43. doi: 10. 1002/jsfa. 5830. Epub 2012 Aug 6. Select item 222582716. Monitoring of gluten-free diet compliance in celiac patients by assessment of gliadin 33 -mer equivalent epitopes in feces. Comino I, Real A, Vivas S, Síglez MÁ, Caminero A, Nistal E, Casqueiro J, Rodríguez-Herrera A, Cebolla A, Sousa C. Am J Clin Nutr. 2012 Mar; 95(3): 670 -7. doi: 10. 3945/ajcn. 111. 026708. Epub 2012 Jan 18. Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease. Comino I, Real A, de Lorenzo L, Cornell H, López-Casado MÁ, Barro F, Lorite P, Torres MI, Cebolla. A, Sousa C. Gut. 2011 Jul; 60(7): 915 -22. doi: 10. 1136/gut. 2010. 225268. Epub 2011 Feb 12. Select item 185095348. Toward the assessment of food toxicity for celiac patients: characterization of monoclonal antibodies to a main immunogenic gluten peptide. Morón B, Bethune MT, Comino I, Manyani H, Ferragud M, López MC, Cebolla A, Khosla C, Sousa C. PLo. S One. 2008 May 28; 3(5): e 2294. doi: 10. 1371/journal. pone. 0002294. Sensitive detection of cereal fractions that are toxic to celiac disease patients by using monoclonal antibodies to a main immunogenic wheat peptide. Morón B, Cebolla A, Manyani H, Alvarez-Maqueda M, Megías M, Thomas Mdel C, López MC, Sousa. C. Am J Clin Nutr. 2008 Feb; 87(2): 405 -14.

Rapid kits for allergens detection on foodstuff and surfaces ü Quick and Reliable ü Results in 10 minutes ü No laboratory equipment required Kit Limit of Detection Aler. Tox Sticks Casein 2. 5 ppm Aler. Tox Sticks ß-Lactoglobulin 2. 5 ppm Aler. Tox Sticks Crustacea 7 ppm (dry) / 33 ppm (wet) Aler. Tox Sticks Peanut 1 ppm Aler. Tox Sticks Hazelnut 1 ppm Aler. Tox Sticks Almond 10 ppm Aler. Tox Sticks Soy 1 ppm Aler. Tox Sticks Egg 1. 25 ppm Aler. Tox Sticks Fish 0. 35 ppm (dry) / 1 ppm (wet)

ELISA Sandwich kits for allergens quantification (48/96 wells plates) Kit Aler. Tox Lupine Aler. Tox Sesame Aler. Tox Mustard Aler. Tox Cashew LOD 0. 2 ppm 1 ppm 0. 2 ppm LOQ 2 ppm Aler. Tox Soy (STI) 16 ppb 50 ppb Aler. Tox Walnut Aler. Tox Peanut Aler. Tox Hazelnut Aler. Tox Almond Aler. Tox Pistachio Aler. Tox Egg Aler. Tox Lysozyme 0. 6 ppm 0. 3 ppm 0. 2 ppm 0. 13 ppm 0. 05 ppm 2 ppb 2 ppm 1 ppm 0. 5 ppm 1 ppm 0. 4 ppm 25 ppb Aler. Tox Ovalbumin 4 ppb 25 ppb Aler. Tox Casein Aler. Tox Milk Aler. Tox BLG Aler. Tox Crustacean Aler. Tox Fish 0. 05 ppm 1. 5 ppb 1. 4 ppb 0. 20 ppm 0. 5 ppm 10 ppb 20 ppb 4 ppb

ELISA Sandwich kits for allergens quantification in wine (48/96 wells plates) Kit Aler. Tox Lysozyme LOD 2 ppb LOQ 25 ppb Aler. Tox Ovalbumin 4 ppb 25 ppb 0. 05 ppm 0. 20 ppm Aler. Tox Casein Method in compliance with O. I. V. requirements (Organisation Internationale de la Vigne et du Vin)

ü Easy to use. ü Rapid results: 2 minutes. ü Simple: it does not require any preparation, calibration, external reagents, or instruments. ü No maintenance required. ü Can be used with warm/hot oil directly or by microwaving cold oil. ü Ideal for seeds oils: sunflower, corn, peanut, soybean, palm olein, coconut oil. Not suitable for olive (green) and palm oil (red).