Bioinformatics Platform Threetier Architecture Static Pages SHTML for

Bioinformatics Platform Three-tier Architecture Static Pages (SHTML) for Web Site Dynamic pages (JSPs) for database driven Entry, Dissemination and Queries Middleware implemented using Entity-Class Operations, Servlets in Java Object-based Relational Database implemented using Oracle - Top-most layer of user interface - Static pages for web site management - Dynamic pages for database driven con - Communicates with database and user - Keeps the complexity of the database Invisible from user interface - Enables seamless acquisition and dissemination of data - Entity Relationship data model - Ontology Diagram Part of CFG - Implementation using Oracle database overall ontology map

Data Integration Overall Schema for CFG Data Objects

Data Integration Approach to Functional Glycomics Glycan profile of spleen of Fuc. T-VII KO mice with differences in histology staining

Data Integration Approach to Functional Glycomics Expression of glycan related genes in wildtype mouse spleen

Molecule Page Interface Comprises of three main components Data from Cores Automated Acquisition Data from Public databases, links to Public resources Interface to CFG resources and data Molecule Information Portal Expert Contribution Filling out fields as experts on the

GBP Molecule Page Galectin family chosen

Galectin-3 Molecule Page of Galectin-3: General Tab Galectin-3 selected

Galectin-3 Molecule Page Reference Tab: Links to Public Databases

Galectin-3 Molecule Page Genome Tab Link to CFG Transgenic mice

Galectin-3 Molecule Page Proteome Tab Domain Organization

Galectin-3 Molecule Page Link to glycan structure corresponding to high affinity ligand of GBPLink to CFG Glycan Array Glycome Tab Data Select High Signal Spo Link from high affinity glycan to GBP molecule

Galectin-3 Molecule Page Biology Tab Immunology Data Histological Staining Data Metabolism Data Links to CFG Phenotyping data on Galectin-3 KO mice