Bioengineering the Future with Shell Oil Transport Fuels

Bioengineering the Future with Shell Oil

Transport Fuels 75% of liquid fuels are used for transport. 70% of that in cars. Retail fuel is 95% fossil alkanes and 5% from a biological source. Wood Image By 2020, global demand for bio-derived fuels will be 500 B litres.

The Exeter-Shell Projects Bacterial Population Sampling for HC Production High-throughput Screen Development Alkane Production & Secretion in Vibrio furnissii Forced Evolution & Mutagenesis for Increased FA Molecular & HC Analysis Of Isolated Mutants Molecular Control of HC Production & Secretion in Botryococcus braunii B. Braunii Genome Evolution Development of Highthroughput Mutant Analysis and Wax Alkane Synthesis in Inducible Wax Synthesis Imaging Mycobacterium Genes for Alkane Production in In Salt Cress Higher Plants Gene & Pathway Discovery Molecular Bioinformatics Rhodosporidium toruloides Genome Analysis HC Analysis Molecular Control of Hydrocarbon Synthesis & Secretion in Bacteria, Yeast and Algae B. braunii-Bacterial for Advanced Biofuel Production Interactions Alkane Synthesis in Desulfovibrio Metabolism & Enzymes Alkane Synthesis in cyanobacteria FA Catabolism in cyanobacteria HC Synthesis Screen in Oleaginous Yeasts Botryococcus braunii Culture Systems Productivity & HC Extraction Culture Systems & DSP Synthetic Biology Synthetic Ecology for Optimum Algal Growth Variable Chain Length & Branched HC in Fermentative Microbes C-Flux Engineering In Yeast

Synthetic Biology ILV ilv. E Keto acid bkd. AA & bkd. AB MDHLA bkd. B aco. L Engineer the production of gasoline, jet-fuel, diesel and lubricant oil in E. coli that are identical to fossil fuels. Acetyl Co. A acc A to acc D fab. H Fatty acyl. ACP Keto acyl Co. A Malonyl -ACP fab. HB tes. A lux. D Branche d fatty acyl-ACP lux. D FFA FATB 1 Biological Alkanes require no alteration of current engine technologies. lux. C & lux. E F-al Np. AD Alkanes & alkenes FATB 1

“Third Generation” Biofuels from Algae Renewable. Sustainable. Productive. CO 2 Mitigation. Experimental.

Botryococcus braunii Oils. Synthesises and secretes very long chain hydrocarbons. Major fossil component of oil wells.

Botryococcus braunii Research. Genome sequencing and bioinformatics to elucidate oil biosynthetic pathways & interactions with symbiotic bacteria. Quantify B. braunii physiology for Life Cycle Analysis. Model and test oil production in industrial culture conditions.