Bioengineering Teaching Lab Setup By Dr Mirjana Pavlovic

Bioengineering Teaching Lab Set-up By Dr. Mirjana Pavlovic

Why: in general? l l l l As continuation/extension of 12 years development of MS Bioengineering program Frequent critique/demand in SPOTs (student request) Already developing Research Bioengineering Labs Development of undergraduate Bioengineering program Member of Steering Committee for development of that program Dissertation Supervisory Committee Member for three Graduate Students, which will be acting in support to train other students taking the course in hands-on. So, the money invested by students will come back to them as the investment in teaching and elevation of their practical knowledge.

What will new Technologies bring? l l The highest possible and almost perfect merge of life science/medicine and engineering (electrical, mechanical, optical, etc) Learning about them and how to use them, students will highly benefit through conceptualization of their own innovations. I believe it is our collective responsibility as a College to provide an avenue to better train and develop our students to make them competitive with students in other schools in United States and parts of the World, especially in this case that the students are actually the ones demanding for such an opportunity.

The ultimate goal? l In summary, we expect that offering these skills early will allow students to gain experience in their field early so they become better optimal candidates when applying for jobs or into top-tier graduate programs. l That is what this teaching lab would have as the ultimate beneficial effect/goal.

l Colleges and students affected Number of student A. Particularly Bioengineering Courses such as: On average basis there was about 20 students in groups 1, 2, 3 per semester (~60 per year), 6 in DIS group, and 30 in Summer Campus for High School training. Without undergraduate group which is to be started some time it is about 300 students within 3 years. 1. Introduction to Bioengineering (core): BME 5000 As for faculties, it will be me and at least 6 young faculties that are developing their research labs, now (7). They will also be involved in teaching. 2. Tissue Engineering: BME 6334 3. Stem Cell Engineering: BME 6324 4. DIS Non-Thesis Masters : BME 6905 5. Summer campus High School young Talents: EGN 1935 -003_2016 R_61412 B. College of Engineering and Computer Science 1. EEL and Computer Science 2. Mechanical Engineering 3. Ocean Engineering C. College of Science 1. Biochemistry 2. Biology D. College of Medicine

Summary Setting up this lab will also reinforce and capture 3 of the 4 research advancement and strategic plans for the period between 2015 – 2025 mentioned by President Kelly’s during the “State of the Campus” address last year: Healthy ageing (Stem and cancer cell research); Neuroscience (Molecular and Cellular aspect); Ocean Science (Marine drugs testing and effects). SENSORS?

Countess II FL Automated Cell Counter Website Link: https: //www. thermofisher. co m/us/en/home/lifescience/cell-analysis/cellanalysisinstruments/automated-cellcounters/countess-ii-flautomated-cell-counter. html Video Link: https: //www. youtube. com/w atch? v=k. Lm. L 1172 zg. Y

Zoe™ Fluorescent Cell Imager Website Link: http: //www. bio -rad. com/en-us/product/zoefluorescent-cell-imager Video Link: https: //www. youtube. com/w atch? v=Kw. VBg. Ug. SX 7 c

XFp Extracellular Flux Analyzer Website Link: http: //www. agilent. com/enus/products/cell-analysis(seahorse)/seahorseanalyzers/seahorse-xfp-analyzer Video Link: http: //www. agilent. com/enus/products/cell-analysis(seahorse)/energy-pathways

Magnetic Bead Cell Sorter (MACS) Website: http: //www. miltenyibiotec. com/en/ products-and-services/macs-cellseparation/macstechnology/microbeads_dp. aspx? export. PF=1 Video Link: https: //www. youtube. com/watch? v =JQQl. XL 0 O 4 uw

Budget ITEM COMPANY QUANTITY COST Zoe Fluorescent Cell Imager Bio-Rad Technologies 1 $ 7, 495 Countess II FL Cell Counter Thermofisher Scientific Magnetic Beads Cell Sorter (MACS) Miltenyl Biotec XFp Extracellular Flux Analyzer (Agilent Technologies (Seahorse Biosciences) Total (Promo Code: ZOE 4 LESSUS) 1 (Plus consumables & Evos Fluorescent light cubes) 1 (Mini & Midi. MACS Starting Kits) 1 (Plus Consumable & Kits) $ 12, 456. 10 $2080 $ 58, 000 $80, 031 (w/o XFp = $ 22, 031)

Dimensions l DIMENSIONS AND WEIGHT OF INSTRUMENTS l 1. Zoe Fluorescent Cell Imager: Catalog # = 145 -0031 (Bio-Rad) Instrument Dimensions (W x D x H) = 33 x 32 x 30 cm (13 x 12. 6 x 11. 8 inches) Instrument Weight = 9 kg (19. 8 lb) l l l l l 2. Countess II FL Cell Counter: Catalog #: AMQAF 1000 (Thermofisher Scientific) Instrument Dimensions (W x D x H) = 228. 6 x 139. 7 x 228. 6 mm (9 x 5½ x 9 inches) Instrument Weight = 8 lb 3. XFp Metabolism Analyzer: Catalog #: S 7802 A (Seahorse Bioscience) Instrument Dimensions (W x D x H) = 43. 0 cm x 30. 3 cm x 56. 8 cm (16. 9˝ x 11. 9˝ x 22. 4˝) Instrument Weight = 14. 7 kg (32. 5 lb) 4. Magnetic Microbeads MACS (Mini. MACS & Midi. MACS Starting Kits) Cat #: 130 -091 -632 (Miltenyl Biotec) Weight of the Mini. MACS Separator = 80 g Weight of the Midi. MACS Separator = 300 g Dimension (Size) of the Multi. Stand (W × D × H) = 240 × 205 × 210 mm Weight of the Multi. Stand = 2. 15 kg

RESEARCH PROJECT BEHIND- GENERAL IDEA: TO DEVELOP BIOMAGNETISM RESEARCH AT FAU We are working on the project that we could tentatively entitle: Physical methods for targeting Cancer Stem Cells. This is a new and evolving field of research within which we want also to see if the leading theories of Cancer (Cancer Stem Cell Theories and Metabolic Theory of the cancer) work. We shall have the entire range of electromagnetic irradiation, but at this point we are treating them with low frequencies. It is confirmed through some initial experiments that low frequency irradiation cause targeted death of cancer stem cells by disrupting their membranes. Our hypothesis is that physical, targeted cancer stem cell therapy is possible due to specificity of resonance between particular electromagnetic field and magnetic field around cancer stem cell membrane which creates distension in the membrane of this cell as underlying mechanism of its death. This implicates the differences in the structure of normal cell, cancer stem cell and other cells in the tumor which are considered non-tumorigenic. l According to new theories of cancer stem cells, beside striking functionality of tumorigenesis and metastatic capacity, cancer stem cells have specific extra or intra-cellular markers which are clonally (clonal mechanism theory) or mutationally (hierarchical theory) perpetuated through the cell without any changes. Within last decade many of them are found and helped the development of the cancer stem cell origin of the tumor (Dick, 1997, AL-Hajj, 2004, Li, 2007 e. t. c. ). l This concept has also assisted in development of Targeted Cancer Stem Cell Therapy. Therefore, we are using Prostate Cancer Stem cell as the subject of low frequency targeted electromagnetic treatment in expectation to get detrimental cellular effect. This model has a CD 44+ marker for cancer stem cell tumorigenicity. (Functionality and phenotype have to work together. ). l 1. Saheed's abstract: Cytostatic and Metabolic Effects of Electromagnetic Field Therapy on Prostate Spheroid Forming Cells (Prostate Cancer Stem/Cells). l 2. Evans abstract: Sequencing of specific marker from isolated prostate CSCs l 3. Mazhar's abstract: Adaptive Role of weak magnetic fields in Modulating Metabolic Pathways in Spheroid forming Prostate Cancer Cells/Prostate Cancer Stem Cells.