Biochemical Engineering Subtext Biochemical Engineering Shigeo Katoh and

Biochemical Engineering Sub-text Biochemical Engineering Shigeo Katoh and Fumitake Yoshida Wiley-VCH 2009 Print-outs (Web site)

Bioproducts in Japan (2008) Product Amount(billion US$) (%) Foods 45 alcoholic beverage 34 seasoning(soy, miso --) 2. 6 fermented food 4. 6 bread 3. 6 Other foods 4. 5 sweetener 1. 1 amino acid, peptide, vitamin 1 enzymes 0. 5 Medicine 9. 3 antibiotics 0. 7 enzyme, amino acid 1 bio-derived protein 1. 2 recombinant medicine 2 antibody 1. 3 diagnostic product 1. 2 Chemicals 3. 6 cosmetics, detergent -- Ratio of New Biotech(%) 62 0. 8 6 10 13 67 5 54

Scheme of bioprocess

Production of interferon by recombinant E. coli

Microorganisim (Escherichia coli)

Fermentor (Bioreactor)

Downstream Processes

Scheme of bioprocess

Biology Microbiology Biochemistry Genetic Engineering Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control Bioseparation Cell separation Adsorption Chromatography Scheme of bioprocess

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

Heat and mass transfer film transfer coefficient overall transfer coefficient estimation of transfer coefficient heat exchanger mass transfer equipment Enzyme reaction Michaelis-Menten equation evaluation of kinetic parameters

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

What are proteins ? How are proteins synthesizes ? Protein production in recombinant cells

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

Cell growth kinetics batch culture fed-batch culture continuous culture production of target batch culture continuous culture

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

Fermentor design operation mode stirred tank reactor power requirement oxygen transfer rate bubble column reactor oxygen transfer rate fermentation strategy fed-batch culture fermentor reservoir V (L) continuous culture Feed medium ＋ Trace metal solution

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

Cell ceparation filtration centrifugation membrane separation

Biology Microbiology Biochemistry Genetic Engineering Week 1: Course introduction and Basic concepts (Chaps. 1 & 2) Chemical Engineering Thermodynamics Transport phenomena Reaction engineering Process control Biochemical Engineering Cell growth kinetics Reactor design Fermentation strategy Process control 2: Heat and mass transfer (Chaps. 5 & 6) Bioseparation Cell separation Adsorption Chromatography 8: Cell separation and other separation methods (Chaps. 8 - 10) 3: Fundamental reaction kinetics and enzyme reaction (Chap. 3) 4: Fundamentals of biochemistry and genetic engineering (OHP) 5: Cell kinetics and mode of fermentor operation (Chap. 4 & 12. 6) 6: Bioreactors and fermentor design (Chap. 7) 7: Bioreactors and fermentation strategy (Chap. 7 & OHP) 9: Adsorption and chromatography (Chap. 11 and OHP) 10: Adsorption and chromatography (Chaps. 11, 13 and OHP) EXAM

Separation processes in bioindustry adsorption chromatography membrane separation application of immunointeraction antibody chromatography C time