BIOC 201 By Lateefa M F Lab Safety

BIOC 201 By Lateefa M. F.

Lab Safety

General Safety Rules 1 -Always Wear a lab coat the best protection against chemical spills and fire.

General Safety Rules 2 - Wear a gloves 3 - Wear safety goggles to protect your eyes from chemicals, heated materials, or things that might be able to shatter.

General Safety Rules 4 - Tie back long hair.

General Safety Rules 5 - Listen to or read instructions carefully before starting to do anythings. 6 - Do not work in the lab unless your instructor is present to supervise your work.

General Safety Rules 7 - Know the location of the fire extinguisher, fire blanket, eyewash station, and first aid kit.

General Safety Rules 8 - Never “horse around” or play practical jokes in the lab.

General Safety Rules 9 - Do not Eat or drink in the lab.

General Safety Rules 10 - After handling chemicals, always wash your hands with soap and water.

General Safety Rules 11 - Keep sinks clean! 12 - Clean up your lab area at the end of the lab period.

Chemical Safety 1 - Never mix chemicals together unless you are told to do so 2 - Do not test any chemical in the lab (Many chemicals are toxic or corrosive. 3 - Do not keep food or drink items at your lab bench. 4 - Avoid skin contact with any chemical.

Chemical Safety 5 - Never pipet by mouth (use arubber bulb or pipet pumps). 6 - Never pipet directly from a reagent bottle. (transfer by beaker) .

Chemical Safety 7 - Do not inhale reagent fumes. (Use a waving motion of your hand to bring the vapor near your nose. ( )

Chemical Safety 8 - Never pour water into a concentrated acid. Always Add Acid ! Always Pour Down a Stir Rod!

Chemical Safety 9 - Use the fume hood when directed to do so. Work with concentrated acids, or with bromine, chlorine, or hydrogen sulfide should be done only in a fume hood 10 - Be sure youe hands and your lab area are dry before using electrical equipment.

Chemical Safety 11 - Don’t Use Broken or cracked glassware 12 - Broken glassware should be disposed in a special container for it.

Heating Safety 1 - Use tongs or protective gloves to handle hot objects

Heating Safety 2 - The only type of glassware that may safely be heated is either Kimax or Pyrex. 3 - Use a wire gauze when heating Beakers.

Heating Safety 3 - Point Test Tubes Toward the Wall and Move it Around When Heating!

Heating Safety 4 - Get a Small, Blue Flame by Turning the Barrel, Regulating the Air Mixture

Beaker ﻛﺄﺲ ﺯﺟﺎﺟﻲ The beakers are borosilicate glass (heat resistant) and graduated along the sides for measurement. These are perfect for heating liquids and storing solids in the laboratory.

Flask ﺍﻟﺪﻭﺭﻕ ﺍﻟﻤﺨﺮﻭﻃﻲ flasks are commonly used for simple measuring, storing and mixing of liquids. They are of similar to beakers but less than graduated cylinders.

Pipets ﺍﻟﻤﺎﺻﺎﺕ Pipets are used to measure and transfer small volumes (10 m. L or less) of liquids. Pipets are long graduated tubes that allow one to measure and transfer small volumes.

Pippet Bulbs ﻣﻀﺨﺔ ﻣﺎﺻﺔ The orange pipette bulb can be used with the 10 ml pipet. The thumbwheel pipetter is designed for use with the 2 ml pipettes but will also work with small sizes. Neither work with corrosive liquids.

Dropping Pipet ﺍﻟﻘﻄﺎﺭﺍﺕ Used to transfer liquids in qualitative test. It does not allow for measurement

Test tubes ﺍﻧﺎﺑﻴﺐ ﺍﻻﺧﺘﺒﺎﺭ A glass test tube is the most common of lab supplies. Made from for borosilicate glass strength & heat resistance. Test Tube Brush Test tube rack Test Tube Holder

ﺩﻭﺭﻕ ﻋﻴﺎﺭﻱ Measuring flasks

Microscope ﺍﻟﻤﺠﻬﺮ A microscope is an instrument for viewing objects that are too small to be seen by the naked or unaided eye

Scale ﺍﻟﻤﻴﺰﺍﻥ an instrument or machine for weighing.

Common Safety Symbols Flammable Explosive Oxidizer ����������

Common Safety Symbols Corrosive Poison Environmental Hazard ������ ����

IN CASE OF ACCIDENT 1 - If a chemical splashes into your eye, get help immediately. Shout out, "I have chemical in my eye!" Use Eyewash Station if Needed…Hold Open Eyes and Press Water On.

IN CASE OF ACCIDENT 2 - Any chemical that comes in contact with your skin should be washed off with water right away. Flush with large quantities of water. For acid spills, apply baking soda solution. For base spills, apply vinegar or boric acid.

Lab Safety Conclusion… l Read Directions! l Use Common Sense! l No Playing Around! l Clean Up! l Keep Sinks Clean! l Put Equipment Back in Right Spots on Carts! l Have Fun…and Learn!

Tools you need in the lab … The lab coat The test tubes The test tube holder The test tube brush The beaker (250 ml) The sticker label The small towel (tissue paper)