BIO 225 Microbiology l l Ken Malachowsky FlorenceDarlington

BIO 225 Microbiology l l Ken Malachowsky Florence-Darlington Technical College

MICROSCOPE Illuminates Magnifies Contrasts SPECIMEN Optics Resolves Distinguishes Lens systems Vary Light intensity Condenser OCULAR OBJECTIVE Lamp Background Distinguish Ability to Provide sharp, crisp image Total magnification Iris diaphragm Multiply two powers Two close objects

Leewenhoek’s Simple Microscope

A Compound Brightfield Microscope

Scanning Power X 4 x

Low Power X 4 x X 10 x

High Power X X 4 x 10 x 4 x 40 x 100 x Field of Vision

Depth Perception Steve Berg winona state university, used with permission

Refraction

The use of Immersion Oil

Microscope Rules • Never force anything on the microscope • Inform the instructor if there is a problem with the microscope • Only use your assigned microscope • Please do the following when you are finished – Click the 4 x objective in place – Lower the stage far from the objective – Place the microscope in its assigned space

Slides to View • Letter E • Colored Threads • Cheek cells • • Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria • Pond water wetmount KM

Cheek Cells 100 x 400 x KM

Slides to View • Letter E • Colored Threads • Cheek cells • • Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria • Pond water wetmount KM

Cyclops 100 x KM

Daphnia 100 x KM

Volvox 100 x 400 x KM

Spirogyra 400 x KM

Amoeba 100 x 400 x KM

Paramecium 100 x 400 x KM

Representative algae, or plantlike protists: The diatom Campylodiscus hibernicus These protists usually have cell walls surrounded by a loosely attached test that contains silicon or calcium carbonate. Most reproduce by binary fission. Some lack flagella.

Diatoms 400 x KM

Mixed Bacteria 1000 x

Slides to View • Letter E • Colored Threads • Cheek cells • • Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria • Pond water wet mount