Berry Full of DNA Exploring Properties of Strawberry
Berry Full of DNA: Exploring Properties of Strawberry DNA Honors Coordinated Science II Wheatley-Heckman
Background • Every living thing contains cells, and these cells contain DNA. • Using the right procedures, we can extract DNA from the cells of almost any living thing. • Examples: The Human Genome Project, Genetic Testing, Crime Scene Investigations… • http: //learn. genetics. utah. edu/content/la bs/extraction/
Why Strawberries? • While human cells contain 2 sets of DNA…strawberry cells contain 8!! That is A LOT of DNA, and it is easy to see when extracted. • Strawberries are easy to mash, and ripe strawberries produce enzymes that contribute to the breakdown of cell walls.
Procedure Overview • First, you will break the cells apart by physically crushing the strawberries in a bag.
Procedure Overview • You will use an extraction buffer (detergent solution) to dissolve the membranes of the cell. • Then, you will filter the dissolved cells using a funnel and cheesecloth to remove unwanted particles. • Ethanol is used to observe the DNA.
Important Notes • Before beginning the lab, answer all the pre-lab questions. • Read the procedure carefully before starting the lab. • Wear safety goggles! • Each table should work as 2 groups. • When finished with the lab, answer all the analysis questions.
What did we see?
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