BENZIDINE TEST BENZIDINE TEST What is meant by

BENZIDINE TEST

BENZIDINE TEST What is meant by benzidine test? Ø is a test to detect the presence of blood at the scene of crime Ø is a presumptive test to detect blood

What is Benzidine? � � � Benzidine is a manufactured chemical does not occur naturally It is a crystalline solid that may be grayishyellow, white, or reddish-gray Benzidine was used to produce dyes for cloth, paper, and leather. It is no longer produced or used commerically in the U. S

Apparatus and Chemicals Required � � � Test Tube/ Filter Paper Gloves Droper Benzidine dihydrochloride Glacial acetic acid hydrogen peroxide

Benzidine Test Procedure and Observations Take 3 ml of fresh benzidine reagent in a test tube. � Add 2 ml of sample in it. � And also add 1 ml of 3% hydrogen peroxide in it. � Observations: A blue or green colour will develop if haemoglobin is present in the sample. This mean blood is present �

Precautions to be taken � � � Before start performing experiment make sure to wash the apparatus. As always, carefully handle all the chemicals. Avoid sample touching with hands while doing the experiment. Use test tube holder for holding test tube. The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then.

Strengths of Benzidrine test � � � An easy test Can be performed by any person /IO Save valuable initial time during investigation

Drawbacks of Benzidine Test � � It Is a presumptive test only Benzidine is a carcinogenic substance

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