BDNF Induces Latephase LTP of C fiber Evoked

BDNF Induces Late-phase LTP of C -fiber Evoked Field Potentials in Rat Spinal Dorsal Horn

“It is easier to find men who will volunteer to die, than to find those who are willing to endure pain with patience. ” ----Julius Caesar

Are We Lost? • Background – LTP and BDNF • Background Some More – Anatomy and Pain • Results and Interpretations – THE Paper • Concluding Conclusions

LTP: It Potentiates…Or Does It? J/K, It Does. Pretest Posttest Treatment Bliss and Lomo, 1973

It’s Never Too Early to be Late • Early phase (E-LTP) – Independent of protein synthesis – Lasts up to 3 hours • Late phase (L-LTP) – Protein synthesis involved – Lasts longer than 3 hours, up to entire life • SUPER DUPER EXTREMELY SUBJECTIVE!!

LTP is the BMOC aka The Leo Da Vinci of Neuroscience

Hi, My Name Is: BDNF X X

Hi, My Name Is: BDNF

Let’s Learn Anatomy!

Anatomy is FUN! Dorsal root and rootlets Spinal (dorsal root) ganglion Ventral root and rootlets Spinal nerve Dorsal ramus Ventral ramus Netter 169

It’s All Fun and Games Until Someone Gets Their Face Burned Off • Nociception: detection of pain – 2 types: mechanical and polymodal • Different signaling molecules • Different molecules enhance response Principles of Neural Science

It’s All Fun and Games Until Someone Gets Their Face Burned Off Principles of Neural Science

It’s All Fun and Games Until Someone Gets Their Face Burned Off • Central sensitization • Two main pathological pain states – Hyperalgesia – Allodynia

LTP Makes it Painful Sandkuhler, J. , Molecular Pain.

Sometimes, a Little Doesn’t Go a Long Way Pezet, S. et al. , Brain Research Reviews.

All’s I Need is a Match, a Pen, and a Compass • Adult male Sprague-Dawley rats • Left sciatic or sural nerve was dissected – Utilized hook electrodes for stimulation – Field potentials recorded – Both HFS and LFS

Um…I Might Need a Map • Spared nerve injury (SNI) – Peroneal and tibial nerves cut • Mechanical sensitivity through von frey hairs • Drugs!!

Look at All the Pretty Squiggly Lines and Dots!

Meh.

I Was in Track in High School…As the Waterboy

“You Got Your Freshmen, ROTC Guys, Preps, J. V. Jocks, Asian Nerds, Cool Asians, Varsity Jocks, Unfriendly Black Hotties, Girls Who Eat Their Feelings, Girls Who Don't Eat Anything, Desperate Wannabes, Burnouts, Sexually Active Band Geeks, the Greatest People You Will Ever Meet, and the Worst. Beware of Plastics. ”

Last, But Not Least

What Color is Your Mood? • rh. BDNF LTP in spinal dorsal horn C-fibers • Blocking Trk. B affected BDNF- and LFSinduced LTP – BDNF differentially involved in LTP stim patterns? • Inhibiting protein synthesis prevented BDNFinduced LTP

What is Your Motivation? • • BDNF-LTP NMDA-dependent BDNF occluded further potentiation in L-LTP Blocking NF-k. B prevented BDNF-LTP Inhibiting ERK and p 38 MAPK no BDNF-LTP – P 38 MAPK and JNK prevents LTP in injured rats • Needed for initiation and maintenance of neuropathic pain in DH

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