BD Probe Tec ET System 1 High Throughput

BD Probe. Tec™ ET System • • 1 High Throughput – 552 test results per 8 -hour shift Excellent reliability – Only 1 moving part – MTBF of over 2 Years Simultaneous amplification and detection Closed, solid barrier amplification reaction system Isothermal Low maintenance Efficient workflow LIS capable

BD Probe. Tec™ Reagents • Dry lyophilized reagents • Room temperature storage • 96 -well microtiter plate format 2

BD Probe. Tec™ ET System • The only “CLOSED, SOLID BARRIER” amplification reaction system on the market – Solid (plastic seal) barrier to amplicon contamination – Never opened or accessed after the start of amplification 3

Instrumentation and Footprint BD Probe. Tec™ ET System 10 - 13 feet total linear footprint* * 13 4 feet with the CA 6000 Centrifuge.

Workflow Overview 5 Sample preparation. Specimens are incubated for lysis for 30 minutes. Specimens are cooled for 15 minutes. 150 µL of lysed specimen is added to the Priming plate to reconstitute reagents and then incubates for 20 minutes. Priming plate is incubated with the Amplification plate for 10 minutes (hot start). 100 µL is transferred from the Priming plate to the Amplification plate. Plate sealer is placed onto the Amplification plate. Sealed plate is placed in the BD Probe. Tec™ instrument. Results are available in 60 minutes.

BD Probe. Tec™ ET Pipettor • 8 samples at a time • Programmable – touch of a button • Performs aspiration & dispense steps • Re-chargeable • Uses aerosol resistant tips with filter 6