BC and Me 1980 to 2003 1980 First
BC and Me – 1980 to 2003 • 1980: First Meeting –NMR meeting • 1984: Joined Brit’s lab • 1984 -87: Animal Models – Open Chest Animals: dog and cat • Volume & pressure overload; ischemia; hypoxia – Closed Chest animals: dog • Volume & pressure overload; cardiomyopathy; heart failure; ischemia – Other intact organs: Skeletal Muscle; liver, brain • Increase work load; ischemia • 1987 -2003: Isolated organs, cells & mitochondria
Multiple Technologies • • NMR: 31 P, 23 Na; 1 H; 13 C; 19 F NADH Fluorescence NIR Spectroscopy Other physiological measurements – Piezoelectric crystals: Mechanical function – Laser Doppler: flow in blood vessels; tissue perfusion – Tissue electrodes: Cae 2+, Ke+, Nae+ – ECG, EEG – BP, HR, LVP
World Wide Colleagues • • • • British Connection: John Haselgrove Chinese Connection: Daning Zhang; DJ Wang French Connection: Bertrand Autlier; Suzanne Wehrli German Connection: Manfred Prammer Hungarian Connection: Laci Ligeti; Tomas Ivanics; Aristied Kovach: Kati Megyeri; Laci Deszi Indian Connection: Hari Subramanian; Ramin Mitra Israeli Connection: Avraham Mayevsky; Myer Shinnar Japanese Connection: Shoko Nioka; K Niibori Netherland Connection: Hans van Beek Polish Connection: Maria Erecinska; Krzysztof Wroblewski Russian Connection: Andre Savchenko Ukrainian Connection: Nicola Doliba; Natalya Doliba; Andriy Babsky Uruguayan Connection: Juan Crestanello US Connection: too numerous to list The World: too numerous to list
NMR Spectroscopy – Multiple collaborations • • • Intact in vivo brain Closed Chest Animals: Dog; Cat Open Chest animals: Dog; Cat Perfused Heart Superfused Cardiomyocytes; hepatocytes Superfused Mitochondria – heart, liver
Closed Skull Cat Brain
Closed Skull Cat Brain – 31 P
Open Chest Animal: Dog or Cat
Open Chest Dog – 31 P
Closed Chest Dog – 31 P
Langendorff Perfused In NMR
Isolated Perfused heart – 31 P
Superfusion System Isolated Cells & Mitochondria
Isolated Superfused Cardiomyocytes -31 P
Isolated Superfused Mitochondria- 31 P
Optical Spectroscopy & Electrode System • NADH Fluorescence – Heart – Brain • NIR – Oxygenation • Multiple electrode system – Ca 2+ – K+ – Na+
NADH Fluorescence & NMR
NADH Fluorescence Hypoxia & NE Administration
Multiparameter Analysis: Flow probe; NADH light guide; Balloon occluder
Multiparameter Analysis: Multi-channel Light Guide; Electrodes (Cae+, Ke+, Nae+; Laser Doppler Tissue Flow; CA flow Probe
Multiparameter Analysis: NADH, Laser Doppler Flow; Ca 2 e+, K 2+
Not the End – Just the Beginning • The story continues through BC’s • Many students • Former colleagues • Friends
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