BBL BBL CRYSTAL 1 Miniaturized Biochemical Assays Bacteria

实验八 细菌的鉴定（BBL半自 动鉴定系统） 一、教学要求 了解不同的微生物快速鉴定方法及应用 初步掌握细菌自动鉴定系统 （BBL CRYSTAL）的操 作技术 二、实验原理 1、 Miniaturized Biochemical Assays Bacteria can be identified by specific biochemical products they produce during growth. Biochemical and enzymatic reactions Miniaturized biochemical assays test for these specific products

API- Identification Kits Gram-negative bacteria Staphylococci

2、Immunoassays l 血清学反应（Serological reactions） Agglutination reactions、Precipitation reactions l 免疫标记（immunolabel) Radioimmunoassays (RIA) Fluorescent immunoassays (FIA) Enzyme-linked immunosorbent assays (ELISA) Chemiluminesent Immunoassay(CLIA)、 Time-resolved fluorescent immunoassays（TRFIA） Immunogold labelling

ELISA（enzyme linked immunosorbent assay） The ELISA can be used to detect the presence of antigens that are recognized by an antibody, or it can be used to test for antibodies that recognize an antigen. l Sandwich ELISA

Immunogold labelling techiques l 斑点免疫金渗滤法 l 胶体金标记免疫层析法

3、Nucleic acid based assay l DNA hybridization – followed by detection with l l l Dot blot assay Fluorescence or Chemiluminesence Polymerase Chain Reaction (PCR) – followed by detection with l l l Gel electrophoresis Fluorescence or Chemiluminesence New assay formats under development