Bacterial Motility Hugh B Fackrell 2 Presentation Outline
Bacterial Motility Hugh B. Fackrell
2 Presentation Outline Bacterial Flagella l Location l Function l Structure l Bacterial vs eukaryotic flagella l 12/1/2020
3 Flagellum vs Pilus 12/1/2020
4 Pilus (fimbriae) An appendage of the bacterial cell used for transfer of genetic information l Plural pili l 12/1/2020
5 Pili Transfer Plasmids 12/1/2020
6 Bacterial Flagellum A thin appendage arising from one or more locations on the surface of the cell wall l Cellular locomotion l Moves cell along gradients e. g. glucose l 12/1/2020
7 Types of Motility l Flagellated – Flagelluml coordinated movement l tumbling movement – Axial filament- spirochetes l Gliding- no visible organelles 12/1/2020
8 Location Polar l Subpolar l Lateral l Lophotrichous l Amphitrichous l Periplasmic l 12/1/2020
9 Polar Flagellum 12/1/2020
10 Lophotrichous Flagella two or more flagella at the end of the microbe Photographs show growth of flagella during replication Spirillum volutns 12/1/2020
11 Amphitrichous Flagella l Tufts of flagella at BOTH ends of the microbe 12/1/2020
12 Peritrichous Flagella 12/1/2020
13 Flagellar tufts 12/1/2020
14 Flagellar tufts 12/1/2020
15 Spirochetes have Periplasmic Flagella l Periplasmic flagella are also called the axial filament 12/1/2020
16 Axial filaments 12/1/2020
17 Bacterial Flagellum 12/1/2020
18 Rings of Gram Negative Flagellum M: cell Membrane l P: Peptidoglycan l L: Outer Layer l S: Spacer l 12/1/2020
19 Flagellar Structure (details) 12/1/2020
20 Prokaryotic Flagellum 12/1/2020
21 Flagellar Structures 12/1/2020
22 Assembly of Flagellum 12/1/2020
23 Demonstration of Chemotaxis Capillary tube contains attractant chemical l Attractants: l – Glucose, Maltose, Alanine l Repellents: – Indole, H+, OH-, fatty acids 12/1/2020
24 Flagellar Rotation Clockwise: Tumbling l Counterclockwise: coordinated l Alternate between two rotations l 12/1/2020
25 “Run & Tumble” motility 12/1/2020
26 Regulation of Flagellar Movement 12/1/2020
27 Movement of Flagella Whip l Egg beater l 12/1/2020
- Slides: 27
