Bacillus cereus 1 Bacillus sp characteristics 2 Gram
Bacillus cereus 1
Bacillus sp. characteristics 2 Gram positive rod Aerobic Central, ellipsoidal spores Two different diseases Two different toxins Many similarities to C. perfringens ~65, 000 cases/year; 85 lab confirmed Vegetative cells not remarkably resistant to heat http: //www. schmidtandclark. com/bacilluscereus
Bacillus sp. 3 Over 100 species Six species in “B. cereus group” � B. cereus � B. anthracis � B. thuringiensis (Bt) � B. mycoides, pseudomycoides, weihenstephanensis � B. cereus and thuringiensis are indistinguishable except for Bt toxin production � Top three all highly related as well � Only the first that food microbiology is concerned with
Ecology and reservoir 4 Similar to other spore-forming organisms Widespread, ubiquitous Soils and plants � Vegetables, starch, spices, ~30% positive Meat products and milk (cross-contaminated with Bacillus from environment)
Growth characteristics 5 Fairly generic characteristics Temperature, generally 4 -48 o. C, optimum 2835 o. C p. H for growth 4. 9 -9. 3 (not terribly acid resistant) Salt resistant; up to ~7. 5% Water activity for growth >0. 95 Spore D 100~3 -200 min
Diseases 6 Characteristics Diarrheal Emetic Dose required 105 -107 105 -108 (cells/gram) Toxin production in Small intestine Food Toxin type Protein enterotoxin Cyclic peptide emetic toxin Incubation period 8 -16 h 0. 5 -5 h Duration of illness 12 -24 h 6 -24 h Symptoms Abdominal pain, watery diarrhea, nausea Nausea, vomiting, malaise Food associated Meat products, Fried and cooked rice, vegetables, puddings, pasta, pastry, noodles sauces, milk and milk From Doyle and Beuchat Food Microbiolog products
Characteristics of toxin 7 Emetic Cereulide; cyclic structure, 3 repeats of (D-O-Leu-D-Ala-LO-Val-L-Val) � Resistant to heat, p. H, proteolysis � � Stable after 121 o. C for 30 min p. H 2 -11 Produced optimally at 20 -25 o. C, late exponential to stationary phase Enterotoxins Sensitive to heat, proteolysis, strong acid � Haemolytic enterotoxin (Hbl) has three subunits (B, L 1, L 3) � Non-haemolytic enterotoxin (Nhe); 99% of strains � Cytotoxin K (Cyt. K) � Foods with >106 organisms generally have sufficient toxin
Association with foods (I) 8 Widespread (as expected) Variety of foods associated � Diarrheal disease milk, meats, vegetables, fish � Emetic disease Rice other starchy foods such as potatoes, pasta cheese products
Association with foods (II) 9 Heat processing may select for (as Clostridium) � More common in pasteurized milk than raw, however: Low numbers (< 103 generally) Toxin production not favored in low temperature milk Sweet curdling or bitty cream � “Chinese Rice Restaurant Syndrome” prepared in bulk; spores survive Rice not properly cooled to below 8 o. C Grow and produce emetic toxin
Outbreaks 10 Mighty Taco, Buffalo, NY, 2016 Pellegrino Food Products (Warren, PA)
Staphylococcus aureus 11
Staphylococcus aureus 12 Gram positive cocci Irregular clumps; grape-like Facultative anaerobes Non-spore forming Intoxication; heat stable toxin Organism isn’t dramatically heat stable Mild, short-duration illness; usually due to contaminated food handlers Salt-tolerant (much lower aw than other organisms) Resistant to drying ~240, 000 cases/yr ; estimated only 1/1000 cases get reported
Growth and toxin production 13 Growth Factor Enterotoxin production Optimum Range Temperature 35 -37 7 -48 35 -40 10 -45 p. H 6. 0 -7. 0 4. 0 -9. 8 A: 5. 3 -6. 8 Other: 6 -7 4. 8 -9. 0 Na. Cl 0. 5 -4. 0% 0 -20% 0. 5% 0 -20% aw 0. 98 -0. 99 0. 83 -0. 99 >0. 99 0. 86 -0. 99 Aerobic/ana erobic Oxygen Taken from Adams and Moss, Food Microbiology
Characteristics of toxin 14 10 known enterotoxins (classified serologically) SEA to SEI � No SEF (declassified) � Three SEC’s (SEC 1, SEC 2, SEC 3) � SEA most common in foodborne outbreaks � SED, SEB also but much less frequent � Isolates may produce multiple SE’s � Called an enterotoxin (is technically a neurotoxin) Binds receptors in gut that stimulate emetic response in brain Resistant to proteolytic enzymes; heat (preformed toxin can be resistant to subsequent heating; mushroom outbreak)
Ecology of S. aureus 15 Primarily: � Skin, skin glands, mucus membranes of warmblooded animals � CDC: 25% of skin/nares of healthy humans � Think: skin contact, nasal drippings, sneezing Also: � Animals Mastitis � Dust, (inflammation of mammary tissue) air, food contact surfaces, environment
Foods associated 16 Meats, poultry, egg products, bakery products… Common features (one or more of following): � Poor personal hygiene; foods made by hand � Preparation of foods far in advance � Inadequate cooking or heating of foods � Slow cooling of contaminated foods (large batches) � Prolonged use of warming plates Is a poor competitor with other organisms � Most of these are inhibited by aw of 0. 86 “Keep hot foods hot (>140 o F), cold foods cold
Characteristics of disease 17 Short incubation period, 1 -10 hours; 4 h typical About 1 -5 mg toxin required (approx. 105 -108 cells in food) Nausea, vomiting, stomach cramps; diarrhea sometimes Recovery complete in 1 -2 days Death uncommon; although ~4% of children and elderly by severe dehydration
Outbreaks 18 July 2011, Crowne Plaza Hotel, Portland, OR July 17: 7 guests, vomiting, diarrhea, cramps between 1 ¾ and 3 ¾ hours after breakfast Linked to hollandaise sauce (butter, pasteurized eggs, lemon juice) on eggs benedict Made at 5: 15 am, “held in a stove” until 11 am; no food sample left to test SEA+SEB S. aureus isolated from 2 cases and one chef; PFGE indistinguishable
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