B 65 Animal Genetic Lesson Outline Introduction Phenotype

B 65 Animal Genetic

Lesson Outline • Introduction • Phenotype • Environmental Factors • Natural Selection • Controlled breeding • Two-Breed Cross • Three Breed Rotation Cross • Gene Transfer • • Genetic Engineering Differentiation Alleles Gene Dominance Punnett Square Mendilin Genetics Codominate Genes Additive Expression of Genes

Introduction q q No two animals are exactly alike. Even with twins one may be taller, one may be heavier, or grow faster. The two main factors that contribute to these differences in animals are: The environment. The genetic make up of the animal. Infovets Educational Resources – www. infovets. com – Slide 3

Phenotype q q q The phenotype is the physical appearance of the animal. The genotype is the genetic make up of the animal Both the environment and the genetic make up effect the physical appearance of the animal. Infovets Educational Resources – www. infovets. com – Slide 4

Environmental Factors q q q The quantity and quality of the feed Climate conditions Exposure to parasites and diseases The type of terrain (steep mountains, desserts, irrigated pasture) The producer has a lot of control over the animal’s environment. A producer can also influence, to a lesser degree, the genetic make up of an animal. Infovets Educational Resources – www. infovets. com – Slide 5

Natural Selection q In nature, genetics are passed on through the process of natural selection. The strongest, healthiest, most powerful animal generally spreads its genetics. Animals that are weak may have a poor immune system and are diseased, or may have conformation problems. Generally these animals do not survive long enough to pass on their genetics. Infovets Educational Resources – www. infovets. com – Slide 6

Controlled Breeding q q A producer crosses two parents based on a desired outcome. A tough, dominant, alpha male may not be a desirable trait for domestic animals. Agriculture producers select for traits that have economic importance, such as low birth weight, growth rate, feed efficiency, mothering ability, carcass traits. The economically important traits are influenced by both the environment and the genetic make up of the animal. Infovets Educational Resources – www. infovets. com – Slide 7

Controlled Breeding Programs q q q Outcrossing: Breeding purebred animals with unrelated purebred animals. Cross Breeding: Breeding animals of the same species but of a different breed. Hybrid Vigor or Heterosis: q A biological phenomenon which causes crossbreeds to out produce the average of their parents q Will achieve 15 to 25% immediate increase in yield q The more dissimilar the breeds, the greater the heterosis: (British breed crossed with Zebu breed) Infovets Educational Resources – www. infovets. com – Slide 8

Two-Breed Cross q q Purebred bulls X purebred cows of another breed 8 -10% increase in weaning weight Infovets Educational Resources – www. infovets. com – Slide 9

Two-Breed Backcross or Crisscross q q q Breed A X Breed B = Crossbred calves Crossbred X Breed A or B Charolais Bull X Hereford Cow = Cross X Charolais Yields 67% of maximum heterosis Infovets Educational Resources – www. infovets. com – Slide 10

Three Breed Rotation Cross q q q 3 Breeds (Angus, Simmental, Charolais) Crossbred females bred to purebred bull of breed A Resulting cross mated to purebred bull of breed B Resulting cross mated to purebred bull of breed C Repeat rotation 87% of maximum heterosis Infovets Educational Resources – www. infovets. com – Slide 11

Gene Transfer q q All selection is based on the concept that desired characteristics are passed on from the parents to the offspring. Humans have 46 chromosomes. Each parent contributes 23. A chromosome is a long protein strand on molecules called DNA is made up of segments called genes. Infovets Educational Resources – www. infovets. com – Slide 12

Gene Transfer q q q Each gene is responsible for a particular trait. Genes form a code or a blueprint for how the animal is to be formed. One chromosome (strand of DNA) will attach to another forming a spiral shape called a double helix. Infovets Educational Resources – www. infovets. com – Slide 13

Gene Transfer q q Each half is bound together by substances called nucleotides. There are four main nucleotides: q Adenine q Thiamine q Guanine q Cytosine Infovets Educational Resources – www. infovets. com – Slide 14

Gene Transfer q q Nucleotides are shaped so that each substance can pair with one particular nucleotide. Adenine can only pair with thiamine. Cytosine can only pair with guanine. When cells undergo mitosis and divide, each half replicates itself so two strands exactly alike are formed. (DNA replication). Infovets Educational Resources – www. infovets. com – Slide 15

Gene Transfer q The genetic sequence on the DNA is used as a pattern for how the animal is to be constructed. RNA (Ribonucleic acid) reads the pattern and transfers the information to the rest of the cell. Infovets Educational Resources – www. infovets. com – Slide 16

Genetic Engineering q q Genetic engineering is a technology that allows specific genetic information or traits to be built into or engineered into the genes of a species. In genetic engineering, segments of DNA are cut and spliced into existing DNA, placing new genetic information into the existing DNA. Infovets Educational Resources – www. infovets. com – Slide 17

Differentiation q q As the embryo begins to grow and develop, cells differentiate. Some cells develop into muscle and bone, some into skin and some into internal organs. The process of how cells differentiate is not fully understood. Infovets Educational Resources – www. infovets. com – Slide 18

Differentiation q q q Sex cells called gametes undergo meiosis and only carry one strand of DNA. At conception, chromosome halves from each parent combine to form a paired chromosome. There is almost an infinite number of ways that the genes can be arranged on a strand of DNA. This arrangement determines the make up of the new animal. Infovets Educational Resources – www. infovets. com – Slide 19

Alleles q q Each male gene that controls a specific trait combines with the female gene for the same trait. A pair of genes that control a specific trait are called alleles. If both genes that control a specific trait are alike, they are said to be homozygous. For example, if the male gene for hair color is black and the female gene that controls hair color is also black. Infovets Educational Resources – www. infovets. com – Slide 20

Gene Dominance q q If they are different (black & red), they are said to be heterozygous. In this case one gene will be dominate and determine coat color. Dominant gene = trait overpowers others Recessive gene = must be accompanied with another recessive gene to express trait Infovets Educational Resources – www. infovets. com – Slide 21

Gene Dominance q q q P = polled p = horned Genotype is the genetic make up of the animal. Phenotype is the physical appearance of the animal. If a homozygous horned cow (pp) is mated to a homozygous polled bull (PP), what will the genotypic and phenotypic ratio of the calves be? Infovets Educational Resources – www. infovets. com – Slide 22

Punnett Square Infovets Educational Resources – www. infovets. com – Slide 23

Monohybrid Cross q q A monohybrid cross is an estimation of a predicted outcome for a single trait. If a homozygous horned cow (pp) is bred with a heterozygous polled bull (Pp), what percent of the calves will be polled? Infovets Educational Resources – www. infovets. com – Slide 24

Monohybrid Cross Infovets Educational Resources – www. infovets. com – Slide 25

Monohybrid Cross q What results if two heterozygous animals are mated. Infovets Educational Resources – www. infovets. com – Slide 26

Dihybrid Cross q q A dihybrid cross is a estimation of a predicted outcome for two traits. What results if an Angus bull that is homozygous black and polled (BBPP) is bred with a red shorthorn cow which is homozygous red and horned (bbpp). The bull BBPP can be simplified to BP (black & polled is the only possible contribution for the bull). The cow bbpp can be simplified to bp (red & horned is the only possible contribution for the cow). Infovets Educational Resources – www. infovets. com – Slide 27

Dihybrid Cross Black = 100% Polled = 100% Horned = 0% Infovets Educational Resources – www. infovets. com – Slide 28

Dihybrid Cross q q q Now if two of the offspring which are heterozygous for black/red and polled/horned (Bb. Pp) are mated. How do you do a Punnett square for two heterozyous animals? Use all possible gene combinations. Both the bull and cow are Bb. Pp. What are the possible contributions? BP, Bp, b. P, bp for both animals. (4 x 4 grid) Infovets Educational Resources – www. infovets. com – Slide 29

Dihybrid Cross Infovets Educational Resources – www. infovets. com – Slide 30

Dihybrid Cross q q Black Polled = 9 out of 16 or 56. 25% Black Horned = 3 out of 16 or 18. 75% Red Polled = 3 out of 16 or 18. 75% Red Horned = 1 out of 16 or 6. 25% Infovets Educational Resources – www. infovets. com – Slide 31

Dihybrid Cross q q If a heterozygous bull (Bb. Pp) is mated to a homozygous cow (BBPP). What are the outcomes? Infovets Educational Resources – www. infovets. com – Slide 32

Dihybrid Cross Infovets Educational Resources – www. infovets. com – Slide 33

Dihybrid Cross q q If a (Bb. Pp) bull is mated to a (BBPp) cow. what are the outcomes? Infovets Educational Resources – www. infovets. com – Slide 34

Dihybrid Cross Infovets Educational Resources – www. infovets. com – Slide 35

Mendilin Genetics q q q Paint color is a desirable characteristic of paint horses and is dominate to solid color. If a homozygous dominate stallion is bred with a solid colored mare, how likely is it that a paint foal will result? What if the stallion is heterozygous for paint color? Infovets Educational Resources – www. infovets. com – Slide 36

Codominate Genes q q q Some alleles may have two dominate genes. Shorthorn cattle are red, white or roan. Red shorthorns carry the gene for red coat color RR. White shorthorns carry the gene for white coat color WW. Cattle that are roan or spotted carry a gene for red and a gene for white RW. Both are dominant, creating a spotted or roan colored animal. Infovets Educational Resources – www. infovets. com – Slide 37

Shorthorn: Red X White Infovets Educational Resources – www. infovets. com – Slide 38

The Additive Expression of Genes q q q Instead of a single pair, a number of genes may be added together to produce a single trait. Examples: Milk production is controlled by several genes. q Size and body capacity of the female q Hormone production q Mammary size and function Rate of gain Reproduction Infovets Educational Resources – www. infovets. com – Slide 39

Genetic Mutations q q q Occasionally a defect will happen and genetic traits are not passed on as intended. Example: two headed calves An abnormality is similar to a mutation, only it is caused by something in the environment Infovets Educational Resources – www. infovets. com – Slide 40

Genetic Mutations Infovets Educational Resources – www. infovets. com – Slide 41

Genetic Mutations q q Sometimes genetic mutations can be used to introduce new kinds of species. Polled Hereford Cattle Infovets Educational Resources – www. infovets. com – Slide 42

Determining an Animal’s Sex q q Whether a mammal is a male or a female is determined upon conception. Gamete (sex cell) contains one half of the sex chromosome from the parent. The female chromosome is referred to as XX. When the chromosome divides and half goes to the offspring each half is the same. Infovets Educational Resources – www. infovets. com – Slide 43

Determining an Animals Sex q q q The male chromosome is referred to as XY. And when divided, a gamete will be either X or Y. When the male and female gamete combine they will either be XX female or XY male. What is the probability of a male being conceived over a female child? Infovets Educational Resources – www. infovets. com – Slide 44

What Sex Will the Offspring Be? Infovets Educational Resources – www. infovets. com – Slide 45

Sex Determination Infovets Educational Resources – www. infovets. com – Slide 46