Automated Microfluidic Cell Separator Project Group 16083 Table

Automated Microfluidic Cell Separator Project Group: 16083

Table of Contents � Group Members � Background Summary � Project Statement � Use Scenarios � Stakeholders � Customer Requirements � Engineering Requirements � House of Quality � Project Plan � Risk Management

The P 16083 Group Member Major Role Contact Jay Dolas BME Lead Engineer Alexandra La. Londe BME Microfludics Specialist ael 9930@rit. edu Vincent Serianni II BME Project Manager vcs 7133@rit. edu Tyler Lisec ME Lead Mechanical tjl 9229@rit. edu Ryan Kinney EE Lead Electrical rhk 9387@rit. edu Chris Molinari EE Controls Engineer cxm 4025@rit. edu jrd 8174@rit. edu

Background Summary What is Microfluidics? “Microfludics is the field that studies the manipulation of small amounts of fluids (10 -9 to 10 -18 liters)” “Microfluidics refers to the handling of liquids or gases at a scale generally below 1 mm, where a number of phenomena that are NOT present or not predominant at larger scales can be exploited for numerous purposes”. “The field of microfluidics is in essence multidisciplinary as it combines microfabrication techniques with chemistry and biology”. BIME 489 -01 Dr. Blanca Lapizco-Encinas

Background Summary What is Dielectrophoretics? Dielectrophoresis is the movement of particles due to polarization effects in a non-uniform electric field. In the presence on a nonuniform electric field, one side of the dipole will be in a region with a lower field intensity. This will produce and UNEVEN charge alignment in the particle, inducing it to move toward the regions of greater field strength BIME 489 -01 Dr. Blanca Lapizco-Encinas

Background Summary What Size of Particles? http: //cnx. org/resources/7 b 4 d 03 a 7 fc 1 e 724 f 75950258 ae 6 d 2356

Background Summary Why Sort Cells? Research and Testing Teaching Tool General Medical Purposes http: //www. mdpi. com/1422 -0067/15/10/18281/htm

Background Summary Other Sorting Methods Centrifugation Flow Cytometry Channel Geometry Magnetic Based http: //eshop. eppendorf. ca/upload/product. View/Eppendorf_ 5427 R_high-capacity_centrifuge. jpg

Problem Statement A cell separator is a device that separates cells in a mixture, based upon pre-established criteria (biomarkers, size, electrical characteristics, etc. ). This is necessary in many cell culture and diagnostic applications where downstream processes occur after cell culture, such as purification or analysis. Optimally, this device should not interfere with the viability or characteristics of the cells, while still being cost effective. Current cell separation devices require some sort of labeling (either fluorescent or magnetic) which is not only costly but can affect cell behavior and mortality. We propose an automated microfluidic system that utilizes developing technologies (dielectrophoretics) to reduce costs drastically while maintaining cell viability. The goals of this project are to develop a system that not only sorts cells without the use of labeling, but also fits within a biosafety cabinet, is self-driven, and is automated (handsoff once the sample is loaded and sequence has started). The expected result is a functional prototype that fits all of the goals above and is suitable for use in a teaching laboratory. The design and prototype must conform to intellectual property and diagnostic laboratory standards so that it may be marketed this as a definitive step forwards in cell separation technology.

Current State Flow Cytometry Fluorescence labeling Laser to excite and identify the cells Additives could alter or damage cells Labeling is expensive http: //www. appliedcytometry. com/flow_cytometry. php Hydrodynamic Cell Separator Inertial forces to separate cell types High shear forces can damage cells Channel design and manufacturing is timely and expensive http: //www. elveflow. com/microfluidic-tutorials/cell-biology-imaging-reviews-andtutorials/microfluidic-for-cell-biology/label-free-microfluidic-cell-separation-andsorting-techniques-a-review/

Desired State � Dielectrophoresis � Uses electric fields to manipulate the cell location in a stream � No need for additives � No added shear force http: //www. elveflow. com/microfluidic-tutorials/cell-biology-imagingreviews-and-tutorials/microfluidic-for-cell-biology/label-freemicrofluidic-cell-separation-and-sorting-techniques-a-review/

Project Goals and Deliverables Working prototype that can: � Demonstrate in a class setting the use of dielectrophoretics in cell separation � Act as a partially automated system � User only has to load the sample and set the target specifications � Maintain cell viability during sorting process � Accurately sorts the target cells Documentation of the prototype that illustrates: � Proper use and care of the device � Target specifications � Voltage amplitude and frequency standards to sort a given cell type

Key Constraints � Device start-up cost below $5000 � Lightweight - able to be moved by 1 person � Electrically shielded and insulated � Bio-hazard containment � Footprint (1. 5' x 1. 5') � 120 V outlet compatible � Reusable channel � Perform process within one hour

Use Scenarios – Teaching Aid

Use Scenarios – Medical Field

Stakeholders Customer – Dr. Blanca Lapizco-Encinas End Users – Lab Workers, Professors, Researchers, Students Potential Sponsors – Rheonix Inc. or the BME Department at RIT Other Stakeholders – P 16083 Group, MSD Team http: //www. rheonix. com/corporate/careers. php https: //twitter. com/ritbme

Customer Requirements Scale: 1 = Less Important, 3 = Moderately Important, 9 = Very Important

Customer Requirements Separation can be visualized Scale: 1 = Less Important, 3 = Moderately Important, 9 = Very Important

Engineering Requirements Scale: 1 = Less Important, 3 = Moderately Important, 9 = Very Important

Engineering Requirements Scale: 1 = Less Important, 3 = Moderately Important, 9 = Very Important

House of Quality n = Less Important n = Moderate Importance n = Most Important

Project Plans MSD I: � Define problem and requirements � Interact with the Customer � Interview, Funding, Customer vs Engineering Requirements � Gather data for design and hardware � Research, Design, Review, Standards, Bill of Materials (BOM) � Validate channel designs and characterize cell behavior MSD II: � Build validated designs � Circuits, PDMS Channels, 3 D Print (where needed), Subsystems � Benchmark device and compare to engineering requirements � Present project in working state � Imagine RIT, Class Demo

Project Plans (MSD I)

Project Plans (First 3 Weeks)

Project Plans (Next 3 Weeks)

Risks

Technical Power Supply Failure Size/Weight Channel Fabrication Errors Fluid Flow Pressure Cells Having Similar Electrical Properties Cell Viability http: //www. micromanufacturing. com/sites/default/files/stories/i mport/1002 -Fluids-web-images/micronitimage_opt. jpeg

Resource Expense of Components Lack of Manpower Data Gathering Cells for sorting Clean Room Workers http: //images. clipartpanda. com/no-money-sign-clip-art-money-sign. png

Safety Electric Shock Lab Work Channel Fabrication Leaks http: //www. emedco. com/media/catalog/ product/Indl-Eyewash-Shower--First. Aid-Signs-42878 BBVPLY 2 WY-ba. jpg http: //www. health-safetysigns. uk. com/productimages /Danger-Electric-shock-risksign. gif

Environmental & Societal Waste Generation Potential Airborne Chemicals Disposal of Chemicals Teaching Laboratory Setting http: //cdn 2. bigcommerce. com/server 4600/10 c 6 f/ products/1621/images/3022/WS 32001_warning _airborne_toxic_chemicals__69205. 1403023543. 1280. png? c=2

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