Autoantibodies against AT 1 Receptor Contribute to Vascular
Autoantibodies against AT 1 Receptor Contribute to Vascular Aging and Endothelial Cell Senescence Wang Meili, Yin Xiaochen, Zhang Suli, Mao Chenfeng, Cao Ning, Yang Xiaochun, Bian Jingwei, Hao Weiwei, Fan Qian, Liu Huirong 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. 2 Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Disease, Capital Medical University, Beijing, China. 3 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. 4 Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China. 5 Beijing Anzhen Hospital, Capital Medical University, Beijing, China. Figure 5. AT 1 -AAs induced premature senescence of HUVECs through AT 1 receptor. A) Representative images and quantitative graphs of p 53, p 21 and p 16 INK 4 a expressions. HUVECs were incubated with AT 1 -AAs-Ig. Gs of indicated concentrations for 5 days. B) Representative images and quantitative graphs of p 53, p 21 and p 16 INK 4 a expressions. HUVECs were incubated with AT 1 -AAs-Ig. Gs (1 &#x 003 BC; M) for indicated times. Aging and Disease, 2019, 10(5), 1012 -1025. DOI: 10. 14336/AD. 2018. 0919 &#x 0002 A; p &#x 0003 C; 0. 01, &#x 0002 A; p &#x 0003 C; 0. 001 vs. the control group. C) Representative western blot and quantitative graphs of p 53, p 21 and p 16 INK 4 a expressions in HUVECs treated with n. Ig. Gs, AT 1 -AA or valsartan plus AT 1 -AA for 72 hrs. D. Measurement of cell proliferation using the cck -8 analysis. HUVECs treated with AT 1 -AA manifested a reduction in cell proliferation by comparison to the n. Ig. G treated group. E) Cell cycle analysis of the n. Ig. Gs, AT 1 -AAs-Ig. Gs or valsartan plus AT 1 -AAs-Ig. Gs-induced HUVECs by flow cytometry. F) Photographs of typical SA-&#x 003 B 2; -gal-stained HUVECs in the n. Ig. Gs, AT 1 -AA and valsartan+AT 1 -AA groups (senescent cells are stained blue). Scale bar = 400 &#x 003 BC; m. H. Quantification of
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