Attenuated measles virus controls pediatric acute Blineage lymphoblastic

Attenuated measles virus controls pediatric acute Blineage lymphoblastic leukemia in NOD/SCID mice by Nike C. Lühl, Felix Zirngibl, Carmen Dorneburg, Jiwu Wei, Meike Dahlhaus, Thomas F. E. Barth, Lüder H. Meyer, Manon Queudeville, Sarah Eckhoff, Klaus. Michael Debatin, and Christian Beltinger haematol Volume 99(6): 1050 -1061 May 31, 2014 © 2014 by Ferrata Storti Foundation

MV-Edm effectively replicates in and kills ALL cell lines (A) Dose-dependent killing of Jurkat

MV-Edm effectively kills cells of pediatric primary ALL and xenografts ex vivo. Nike C.

Hematopoetic cells are not sensitive to MV-Edm despite sufficient expression of CD 46 and

The RIG-I/MDA-5/MAVS/IFN-β axis is defective in ALL cell lines but intact in B-cell precursor ALL xenograft cells. Nike C. Lühl et al. Haematologica 2014; 99: 1050 -1061 © 2014 by Ferrata Storti Foundation

In NOD/SCID mice with B-lineage ALL xenografts MV-Edm eradicates or profoundly decreases peripheral blasts leading to survival of most mice NOD/SCID mice with the common ALL xenografts #15 and #13, the pro B ALL xenograft #6 and the pre B ALL xenograft #19. . . Nike C. Lühl et al. Haematologica 2014; 99: 1050 -1061 © 2014 by Ferrata Storti Foundation

Systemic MV-Edm efficiently replicates, spreads and kills within an ALL xenograft in vivo. Nike

Systemic MV-Edm curbs CNS disease In NOD/SCID mice with ALL xenografts (A) CNS disease is established early. 2 mice with xenograft #21 were killed once peripheral leukemic blasts reached 5– 20 % (90 d), as determined by FACS, and CNS disease was assessed by. . . Nike C. Lühl et al. Haematologica 2014; 99: 1050 -1061 © 2014 by Ferrata Storti Foundation