Atomic Force Microscopy Alexander Chew Florida State University

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Atomic Force Microscopy Alexander Chew Florida State University BSC 5936 April 2005

Atomic Force Microscopy Alexander Chew Florida State University BSC 5936 April 2005

How It Works • • • Invented in 1986 Cantilever Tip Surface Laser Multi-segment

How It Works • • • Invented in 1986 Cantilever Tip Surface Laser Multi-segment photodetector Figure 4. Three common types of AFM tip. (a) normal tip (3 µm tall); (b) supertip; (c) Ultralever (also 3 µm tall). Electron micrographs by Jean. Paul Revel, Caltech. Tips from Park Scientific Instruments; supertip made by Jean-Paul Revel. http: //www. molec. com/what_is_afm. html http: //stm 2. nrl. navy. mil/how-afm. html#imaging%20 modes

Topography • Contact Mode – High resolution – Damage to sample – Can measure

Topography • Contact Mode – High resolution – Damage to sample – Can measure frictional forces • Non-Contact Mode – Lower resolution – No damage to sample • Tapping Mode – Better resolution – Minimal damage to sample 2. 5 x 2. 5 nm simultaneous topographic and friction image of highly oriented pyrolytic graphic (HOPG). The bumps represent the topographic atomic corrugation, while the coloring reflects the lateral forces on the tip. The scan direction was right to left http: //stm 2. nrl. navy. mil/how-afm. html#imaging%20 modes

Approach • In the approach the tip is not yet in contact with the

Approach • In the approach the tip is not yet in contact with the surface • Attractive forces maybe • Repulsive forces definitely – Due to contact – Gives information about the elasticity or stiffness of sample

Retraction • Attractive forces again during the retraction phase – Chemical and/or electrostatic •

Retraction • Attractive forces again during the retraction phase – Chemical and/or electrostatic • Break of attractive forces due to retraction of the tip > characteristic “jump” in force curve

Who Needs It • Vacuum, Air, Aqueous Medium - Mimic Biological Environment • Sub-nanometer

Who Needs It • Vacuum, Air, Aqueous Medium - Mimic Biological Environment • Sub-nanometer resolution • Manipulate Surface with Molecular Precision • Real Time Direct Structure-Function Studies

• 3 -D Surface Topography • Force Measurements in pico-Newton - nano-Newton range

• 3 -D Surface Topography • Force Measurements in pico-Newton - nano-Newton range • May Be Combined Simultaneously With Other Techniques • AFM with Flourescence • AFM with Patch-Clamp

Applications • Study Unfolding Of Proteins • Imagining Of Biomolecules • Force Measurements In

Applications • Study Unfolding Of Proteins • Imagining Of Biomolecules • Force Measurements In Real Solvent Environments • Antibody-Antigen Binding Studies • Ligand-Receptor Binding Studies • Binding Forces Of Complimentary DNA Strands • Study Surface Frictional Forces • Ion Channel Localization

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Atomic Force Microscopy Atomic force microscopy AFM isAtomic Force Microscopy Atomic force microscopy AFM is
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Atomic Force Microscope 1 Atomic Force Microscope AFMAtomic Force Microscope 1 Atomic Force Microscope AFM
FORCE AND PRESSURE FORCE Force Force is aFORCE AND PRESSURE FORCE Force Force is a
Atomic Force Microscopy Studies of Gold Thin FilmsAtomic Force Microscopy Studies of Gold Thin Films
Fluid Interface Atomic Force Microscopy FIAFM D EricFluid Interface Atomic Force Microscopy FIAFM D Eric
Atomic force microscopy AFM Introduction AFM merupakan peralatanAtomic force microscopy AFM Introduction AFM merupakan peralatan
Atomic force microscopy Ji Boldy Outline Motivation MinisurveyAtomic force microscopy Ji Boldy Outline Motivation Minisurvey
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HIGHRESOLUTION IMAGING WITH FORCES ATOMIC FORCE MICROSCOPY KrystynHIGHRESOLUTION IMAGING WITH FORCES ATOMIC FORCE MICROSCOPY Krystyn
Atomic Force Microscopy as a tool to studyAtomic Force Microscopy as a tool to study
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Atomic Force Microscopy Analysis Methods for shortrange highLETAtomic Force Microscopy Analysis Methods for shortrange highLET
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Deblurring in Atomic Force Microscopy AFM images SupervisorDeblurring in Atomic Force Microscopy AFM images Supervisor
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Optical and Atomic Force Microscopy for analysis ofOptical and Atomic Force Microscopy for analysis of
Atomic Force Microscopy Fig2 schematic of AFM operationAtomic Force Microscopy Fig2 schematic of AFM operation