Atomic Force Microscope Gerrit L Heuvelman KirchhoffInstitut fr

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Atomic Force Microscope Gerrit L. Heuvelman Kirchhoff-Institut für Physik Molekulare Biophysik (F 15)

Atomic Force Microscope Gerrit L. Heuvelman Kirchhoff-Institut für Physik Molekulare Biophysik (F 15)

Scanning Probe Microscopy STM: scanning tunneling microscope tunneling of electrons between probe and surface

Scanning Probe Microscopy STM: scanning tunneling microscope tunneling of electrons between probe and surface AFM: atomic force microscope measuring of the force on the probe MFM: magnetic force microscope AFM with magnetical probe SNOM: scanning near-field optical probe is a fiber; tunneling of fotonen microscope y x

STM: scanning tunneling microscope piezoelement tunneling of electrons through air between probe and surface

STM: scanning tunneling microscope piezoelement tunneling of electrons through air between probe and surface e- n. A probe R e - e ee- e- < 1 nm ee- e- x-y stage only conducting material

STM: scanning tunneling microscope Itip piezo-element (changes length at different voltages) n. A R

STM: scanning tunneling microscope Itip piezo-element (changes length at different voltages) n. A R Icontrol ∆I ∆I -> ∆V transfer

AFM: atomic force microscope SFM: scanning force microscope photodiode laser AFM probe scans over

AFM: atomic force microscope SFM: scanning force microscope photodiode laser AFM probe scans over the surface (in contact) probe piezo-element e. g. living cells, chromatin fibers

MFM: magnetic force microscope photodiode laser AFM with magnetic probe magnetic tip piezo-element e.

MFM: magnetic force microscope photodiode laser AFM with magnetic probe magnetic tip piezo-element e. g. hard disc, tape

SNOM: scanning near-field optical microscope fiber metal-coated fiber tip tunneling of photons between probe

SNOM: scanning near-field optical microscope fiber metal-coated fiber tip tunneling of photons between probe and surface sample lens filter shows the amount of light that is absorbed/transmitted for different colors detector e. g. fluorescent molecules

Atomic Force Microscope laser photodiode cantilever z piezo x cantilever tip y

Atomic Force Microscope laser photodiode cantilever z piezo x cantilever tip y

Atomic Force Microscope mirror laser beam photodiode SPM tip fluid in fluid cell fluid

Atomic Force Microscope mirror laser beam photodiode SPM tip fluid in fluid cell fluid out tipholder sample piezo translator O-ring x, y, z piezo translator sample motor control in air and in buffer solutions

movement of the AFM tip along the sample AFM tip superhelical DNA plasmid path

movement of the AFM tip along the sample AFM tip superhelical DNA plasmid path of AFM tip DNA double helix Mg 2+ Mg 2+ negatively charged mica surface

AFM image of a 6. 8 kb superhelical plasmid AFM tip

AFM image of a 6. 8 kb superhelical plasmid AFM tip

AFM image of a nucleosome on a 614 base pair DNA 2 µm x

AFM image of a nucleosome on a 614 base pair DNA 2 µm x 2 µm overview scan zoom of mononucleosome complexes Kepert, F. , Fejes Tóth, K. , Caudron, M. , Mücke, N. , Langowski, J. & Rippe, K. , manuscript in preparation

AFM image of a nucleosome on a 614 base pair DNA 2 µm x

AFM image of a nucleosome on a 614 base pair DNA 2 µm x 2 µm overview scan zoom of mononucleosome complexes Kepert, F. , Fejes Tóth, K. , Caudron, M. , Mücke, N. , Langowski, J. & Rippe, K. , manuscript in preparation

DNA entry-exit angle of nucleosomes

DNA entry-exit angle of nucleosomes

movement of a 600 bp DNA fragment by AFM

movement of a 600 bp DNA fragment by AFM

different levels of chromatin packing A nm 22 nm D B 11 nm E

different levels of chromatin packing A nm 22 nm D B 11 nm E 300 nm nm 300 700 nm C 30 nm F 1400 nm

chromatin structure 400 nm x 400 nm

chromatin structure 400 nm x 400 nm

chromatin structure 400 nm x 400 nm

chromatin structure 400 nm x 400 nm

chromatin structure

chromatin structure

spectrin C-terminal Helix C Helix A Helix B N-terminal molecule that contributes to the

spectrin C-terminal Helix C Helix A Helix B N-terminal molecule that contributes to the mechanical properties, especially the elasticity of the cells measurement of its mechanical stability provides information about the physiological function

stretching spectrin with an AFM 4 repeated spectrin domain cantilever tip surface 1. adhesion

stretching spectrin with an AFM 4 repeated spectrin domain cantilever tip surface 1. adhesion force between cantilever tip and surface 2. dissociation from the folded state to the intermediate unfolded state force 1 2 3 distance 3. dissociation from the intermediate to the total unfolding state

stretching spectrin with an AFM force (p. N) 100 80 60 40 20 0

stretching spectrin with an AFM force (p. N) 100 80 60 40 20 0 -20 0. 20 20 40 60 distance (nm) 80 0. 15 probability 0 0. 10 0. 05 0. 00 0 10 20 30 40 unfolding force (p. N) 50

Atomic Force Microscope 1 Atomic Force Microscope AFMAtomic Force Microscope 1 Atomic Force Microscope AFM
By Owen Flynn Gerrit Rietveld Life Gerrit RietveldBy Owen Flynn Gerrit Rietveld Life Gerrit Rietveld
The Microscope The Microscope An Introduction The microscopeThe Microscope The Microscope An Introduction The microscope
THE MICROSCOPE Invention of the Microscope The microscopeTHE MICROSCOPE Invention of the Microscope The microscope
Microscope MICROSCOPE A microscope is an instrument usedMicroscope MICROSCOPE A microscope is an instrument used
Brightfield microscope Darkfield microscope Phasecontrast microscope Fluorescence microscopesBrightfield microscope Darkfield microscope Phasecontrast microscope Fluorescence microscopes
THE MICROSCOPE Invention of the Microscope The microscopeTHE MICROSCOPE Invention of the Microscope The microscope
Introduction to the Microscope Compound Microscope Dissection MicroscopeIntroduction to the Microscope Compound Microscope Dissection Microscope
The Microscope The Microscope n Simple Microscope oneThe Microscope The Microscope n Simple Microscope one
Microscope Light Microscope Microscope is an instrument usedMicroscope Light Microscope Microscope is an instrument used
LIGHT MICROSCOPE Care of Microscope Carry the microscopeLIGHT MICROSCOPE Care of Microscope Carry the microscope
Microscopes Light microscope Invert Microscope Fluorescence microscope usesMicroscopes Light microscope Invert Microscope Fluorescence microscope uses
THE MICROSCOPE Invention of the Microscope The microscopeTHE MICROSCOPE Invention of the Microscope The microscope
Atomic Force Microscopy Atomic force microscopy AFM isAtomic Force Microscopy Atomic force microscopy AFM is
Atomic Force Microscopy Atomic force microscopy AFM isAtomic Force Microscopy Atomic force microscopy AFM is
Atomic Force Microscopy Atomic force microscopy AFM isAtomic Force Microscopy Atomic force microscopy AFM is
FORCE AND PRESSURE FORCE Force Force is aFORCE AND PRESSURE FORCE Force Force is a
The Atomic Force Microscope as versatile tool inThe Atomic Force Microscope as versatile tool in
Atomic Force Microscope AFM STM makes use ofAtomic Force Microscope AFM STM makes use of
Probing Membrane Electrostatics with the Atomic Force MicroscopeProbing Membrane Electrostatics with the Atomic Force Microscope
Scanning Probe Microscopy Atomic Force Microscope Fabrication andScanning Probe Microscopy Atomic Force Microscope Fabrication and