ATOMIC ABSORPTION SPECTROSCOPY BY DEVIN CLARKE TOMISLAV DREMPETIC

ATOMIC ABSORPTION SPECTROSCOPY BY; DEVIN CLARKE, TOMISLAV DREMPETIC

TABLE OF CONTENTS • • • INTRODUCTION MANUFACTURERS HOW IT WORKS TESTING SAMPLE PREPARATION RESULTS SERVICING AND MAINTENANCE SAFETY FEATURES TRAINING REFERENCES

INTRODUCTION TO ATOMIC ABSORPTION SPECTROSCOPY • ATOMIC ABSORPTION SPECTROMETRY (ORAA SPECTROMETRY) IS AN ANALYTICAL TECHNIQUE THAT MEASURES THE CONCENTRATIONS OF ELEMENTS. • ATOMIC ABSORPTION IS SO SENSITIVE THAT IT CAN MEASURE DOWN TO PARTS PER BILLION OF A GRAM IN A SAMPLE. • ATOMIC ABSORPTION SPECTROMETRY IS USED IN: ENVIRONMENTAL ANALYSIS, CLINICAL ANALYSIS, MINING INDUSTRY AND PHARMACEUTICAL MANUFACTURING PROCESSES.

MANUFACTURERS TO NAME A FEW MANUFACTURERS: • • AGILENT AURORA INSTRUMENTS BUCK SCIENTIFIC GBC SCIENTIFIC

HOW AA SPECTROSCOPY WORKS • MEASURES THE ABSORBED RADIATION OF A SAMPLE, BY DETECTING THE WAVELENGTHS OF LIGHT EMITTED WHEN SUBJECTED TO EXCITATION THROUGH RADIATION. • THIS INFORMATION IS COMPARED TO READINGS TAKEN BEFORE EXCITATION, AND THEN SENT TO A SIGNAL PROCESSING CIRCUIT FOR ANALYSIS AND DISPLAY. • 3 STEPS ARE INVOLVED IN THE PROCESSING OF A SAMPLE: • • DE-SOLVATION • VOLATIZATION VAPORIZATION • 2 MAIN METHODS ARE USED FOR THIS PROCESS: • • • VIDEO FLAME AA SPECTROSCOPY, GRAPHITE FURNACE AA SPECTROSCOPY Diagram showing the layout/process of how the AA spectrometer works (Atomic absorption spectrometry, n. d. ).

TESTING • MANY THINGS CAN BE TESTED WITH ATOMIC ABSORPTION SPECTROMETRYS. OME OF THESE ARE: • ANALYSIS OF BLOOD (IRON IN HEMOGLOBIN), URINE, CEREBROSPINAL FLUID, TISSUE, FINGERNAILS, HAIR, WATER, FOOD, DRINKS, DRUGS AND SO ON.

SAMPLE PREPARATION • VERY LITTLE SAMPLE PREPARATION IS REQUIRED, DUE TO THE ATOMIZATION PROCESS CONVERTING THE SAMPLE INTO FREE ATOMS FOR ANALYSIS. • DILUTION OF THE SAMPLE IS OFTEN REQUIRED USING AN APPROPRIATE SOLUTION. • REFERENCE SOLUTIONS (SAMPLES CONTAINING KNOWN VALUES OF ELEMENTS) MAY BE USED FOR CALIBRATION OF THE DEVICE.

RESULTS FROM TESTING • RESULTS ARE DISPLAYED AS GRAPHS AND CHARTS • GAO, J. (N. D. )

SERVICING AND MAINTENANCE Agilent Recommended Routine Maintenance Checklist for AA Spectroscopy Devices (Cunliffe, 2010).

SAFETY FEATURES • • • FLAME MONITOR AUTOMATIC GAS LEAK DETECTORS AUTOMATIC AIR-N 2 O SWITCHING GAS PRESSURE MONITOR DRAIN TANK LEVEL MONITOR AUTOMATIC FLAME EXTINCTION

TRAINING • AGILENT OFFERS SEVERAL ONLINE SOLUTIONS FOR TRAINING ONAA SPECTROMETRY DEVICES AND SOFTWARE. • AGILENT ALSO OFFERS ANNUAL TRAINING COURSES WITH INTRODUCTORY MAINTENANCE AND SERVICING OF FLAME AA SPECTROMETERS.

REFERENCES • CUNLIFFE, M. (2010). ROUTINE MAINTENANCE FOR ATOMIC ABSORPTION SPECTROPHOTOMETERS. RETRIEVED 5 MARCH 2015, FROMHTTP: //WWW. CHEM. AGILENT. COML/ IBRARY/APPLICATIONS/AA 039. PDF • ATOMIC ABSORPTION SPECTROMETRY (1 ST ED. ). RETRIEVED FROM HTTP: //WWW. KAU. EDU. SAF / ILES/130002/ FILES/6785_ AA AAS. PDF • GARCIA, R. , & BÁEZ, A. (2012). ATOMIC ABSORPTION SPECTROMETRY (AAS) (1 ST ED. ). MEXICO CITY, MEXICO: CENTRO DE CIENCIAS DE LA ATMÓSFERA, UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO. RETRIEVED FROM HTTP: //CDN. INTECHOPEN. COM/PDFS-WM/26275. PDF • AGILENT ATOMIC ABSORPTION SPECTROMETERS. (2014) (1 ST ED. ). RETRIEVED FROM HTTP: //WWW. CHEM. AGILENT. COML / IBRARY/BROCHURES/5990 -6495 EN. PDF • ATOMIC SPECTROSCOPY. (2015) (1 ST ED. ). WALTHAM, MA. RETRIEVED FROM HTTP: //WWW. PERKINELMER. CAC / ONTENT/RELATEDMATERIALS/BROCHURES/BRO_WORLDLEADERAAICPMSICPM S. PDF • GAO, J. (N. D. ). RETRIEVED MARCH 5, 2015, FROM HTTP: //WWW. SCIELO. CL/SCIELO. PHP? SCRIPT=SCI_ARTTEXT&PID S=071797072008000100020&LNG=EN&NRM=ISO&IGNORE=. HTML • AA-7000 ATOMIC ABSORPTION SPECTROPHOTOMOTER. (N. D. ). RETRIEVED MARCH 5, 2015, FROM HTTP: //WWW. BIOCOMPARE. COM/9963 ATOMIC-ABSORPTION-SPECTROMETERS/1066119 AA 7000 -ATOMICABSORPTION-SPECTROPHOTOMETER/