Article Effects of Estrogen Nitric Oxide and Dopamine
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Article Effects of Estrogen, Nitric Oxide, and Dopamine Interactionson Behavioral Locomotor Activities in the Embryonic Zebrafish ‘Listless” Model of Locomotor Dysfunction: A Pharmacological Study Vania Murcia, Luke Johnson, Meredith Baldasare, Bridgette Pouliot, John Mc. Kelvey, Brandon Barbery, Julie Lozier, Wade E. Bell, and James E. Turner Department of Biology, Center for Molecular, Cellular, and Biological Chemistry
OXIDOPAMINE 6 -HYDROOXYDOPAMINE (6 -OHDA) -Neurotoxic synthetic compound -Used by researchers to selectively destroy dopamine and noradrenaline reuptake transporters
WHY ZEBRA FISH? -Because neurotoxin damage to their nigrostriatal-like neurons exhibit motor anomalies similar to those of human patients -Their nigrostriatal-like neurons provide a similarity to human nigrostriatal projections to the striatum of the basal ganglia
WHY ZEBRA FISH CONT. -BG pathway is similar and the DA neurons are fewer in number and easier to visualize compared to human patients -Zebrafish and adults respond to DA neurotoxins and 6 OHDA in a similar way
AFFECTS OF NITRIC OXIDE -NO affects motor function -- acts through regulation of dopamine -When NO is inhibited in zebrafish using NO synthase inhibitor (n. NOSI) a condition called “Listless” occurs -- this is where the fish lack swimming abilities and have difficulties maintaining balance
TREATMENT -Co-treatment with either NO or estrogen can rescue the fish from the “listless” phenotype caused by 6 OHDA -NO deprived zebrafish were rescued from the phenotype when co-treated with LDOPA -The longer fish are exposed to 6 OHDA and n. NOSI co-treatment the slower the recovery compared to single treatment of
RESULTS -The results indicate that there is a link between E 2, NO, and DA systems that regulate motor functions in the embryonic fish
QUESTIONS?
WORK CITED Effects of Estrogen, Nitric Oxide, and Dopamine Interactionson Behavioral Locomotor Activities in the Embryonic Zebrafish ‘Listless” Model of Locomotor Dysfunction: A Pharmacological Study Vania Murcia, Luke Johnson, Meredith Baldasare, Bridgette Pouliot, John Mc. Kelvey, Brandon Barbery, Julie Lozier, Wade E. Bell, and James E. Turner Department of Biology, Center for Molecular, Cellular, and Biological Chemistry Virginia Military Institute, Lexington, VA 2445 0
