Arabidopsis experiment sample identifiers Arabidopsis experiment sample identifiers

Arabidopsis experiment sample identifiers

“Human Cell Lineage Project” 1. 2. 3. 4. 5. Who cares? Many biologists. Is it possible? Theoretically, yes. Is it feasible? Only if fully automated. What next? Look at real trees What next? Controlled experiment in Human tissue culture

Human MMR-deficient tissue culture trees LS 174 T Human Adenocarcinoma High mutation rates in MS

Human MMR-deficient tissue culture trees Out of 135, 135 possible topologies

Amplification: 8 multiplexes Capillary: 4 runs Tissue culture trees procedure

TCT A – Cell identifiers Leaf plates Plate A 1 a identifier Locus 0707 over all plates

Human MMR-deficient tissue culture trees

Human MMR-deficient tissue culture trees B Minimal set: 5994, 2068, VWA 73 B 1 B 2 52 B 1 a B 1 b B 2 a 41 B 1 a 2 B 1 b 1 B 1 b 2 B 2 a 1 B 2 a 2 B 2 b 1 B 2 b 2 27 B 1 a 1 a B 1 a 1 b B 1 b 1 a B 1 b 1 b B 2 a 2 a = -1 @ 5994 (lower allele) = -1 @ 2068 (lower allele) = -3 @ 5994 (upper allele) = -1 @ 2068 (upper allele) = +1 @ 5994 (upper allele) = +1 @ 2068 (upper allele) = +2 @ 5994 (upper allele) = +2 @ VWA (lower allele) = +1 @ VWA (upper allele) Out of 34, 459, 425 possible topologies

Human MMR-deficient tissue culture trees C Minimal set: 1721, 6458, 7509, 6424 64 C 1 C 2 33 C 1 b C 1 a C 2 b C 2 a 51 C 1 a 2 C 1 b 1 C 1 b 2 C 2 a 1 C 2 a 2 31 C 1 a 1 a C 1 a 2 b C 1 b 1 a C 1 b 1 b C 1 b 2 a C 1 b 2 b C 2 a 1 a C 2 a 1 b C 2 a 2 a C 2 a 2 b = -1 @ 1721 (lower allele) = +2 @ 6424 (lower allele) = -1 @ 1721 (upper allele) = -2 @ 6424 (lower allele) = +1 @ 1721 (upper allele) = +1 @ 6424 (lower allele) = +1 @ 6458 (upper allele) = -4 @ 6424 (upper allele) = -1 @ 6458 (upper allele) = +1 @ 6424 (upper allele) = +2 @ 6458 (upper allele) = -1 @ 6424 (upper allele) = -1 @ 7509 (lower allele) Out of 13, 749, 310, 575 possible topologies

Human MMR-deficient tissue culture trees

Actual Cell Lineage Inference Machine @ Ullman 3 XXX

“Human Cell Lineage Project” 1. 2. 3. 4. 5. 6. Who cares? Many biologists. Is it possible? Theoretically, yes. Is it feasible? Only if fully automated. What next? Look at real trees What next? Human tissue culture What next?