Applied Biotechnology Innovation Centre RESEARCH AND TECHNOLOGY TRANSFER

Applied Biotechnology Innovation Centre RESEARCH AND TECHNOLOGY TRANSFER AT THE BIO/NANO/ELECTRONIC INTERFACE Ioanis Katakis Octoner 25, 2004 Tarragona

Applied Biotechnology Innovation Centre WHO ARE WE? Evolution Technicians Students Ph. D of membership 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 BBCAT

Applied Biotechnology Innovation Centre HOW WE FINANCE OUR WORK? private public 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 BBCAT

Applied Biotechnology Innovation Centre HOW WE WORK? Knowledge Creation • • • Biosensors Bioelectronics Nanotechnology Microsystems (Photo)catalysis Biotechnology Innovation Technology Transfer • Clinical & veterinary diagnostics • Biomedical applications • Food & Agriculture • Pharmaceutical & Fine Chemicals • Chemical Industry • Renewable energies • Spin off companies • New educational/vocational models

Applied Biotechnology Innovation Centre OUR SECOND RESEARCH PLAN Enzyme (NAD) BIOSENSORS Immunosensors BIOELECTRONICS DNA Biofuel cells Neuron chip NANOTECHNOLOGY Directed deposition Nanocatalysts MICROSYSTEMS NEW AREAS Aptamers Bioreactors Solar Cells Screen printed Microreactors APPLIED RESEARCH Making immuno strips and systems

Applied Biotechnology Innovation Centre FROM SUB MOLECULAR TO MACRO ECOSYSTEMS ORGANISMS CELLS PROTEINS GENES MICROSYSTEMS BIOELECTRONICS TISSUE ENGINEERING IN VITRO SYSTEMS BIOSENSORS NANOTECHNOLOGY MA T E R I A L S A N D TISSUES MULTI MICRO BIOREACTORS C H E MI S T R Y ORGANS ENVIRONMENTAL BIOTECHNOLOGY

Applied Biotechnology Innovation Centre SOME RESEARCH LINES (1/4) SELF ASSEMBLY FOR THE ULTIMATE (BIO)CHEMICAL CATALYTIC FLEXIBILITY • Narváez et al. Biosens Bioelectr 15: 43 -52 (2000) • Narváez et al. J Electroanal Chem 430: 227 -33 (1997)

Applied Biotechnology Innovation Centre SOME RESEARCH LINES (2/4) SUPRAMOLECULAR ARCHITECTURES FOR COMPLEX BIOSENSING, AMPLIFICATION, AND CATALYSIS TASKS Sonicate, extrude • Popescu et al. J Electroanal Chem 464: 208 -14 (1999) (+2 in preparation) • V. Pavlov Thesis (2005) on biosensors, K. Horton DEA (2003) liposomes

Applied Biotechnology Innovation Centre SOME RESEARCH LINES (3/4) NANOCOLLOID SYNTHESIS AND MODIFICATION FOR NANOPATTERNING AND ARTIFICIAL INTELLIGENCE conjugation gold colloid oligonucleoti de deposition colloidal gold selective deposition oligonucleoti • Campàs & Katakis Int J Env Anal (in press), PCT&US pat. de. Appl (2002) • M. Campàs Ph. D Thesis (2003), S. Dondapati DEA Thesis (2004)

Applied Biotechnology Innovation Centre SOME RESEARCH LINES (4/4) TOWARDS THE ULTIMATE NANOMACHINE(? ): SELF POWERED, SELF PROPELLED, SELF PROPAGATING e. Os+ - Os+ Os+ - + -}N 2 + + - ++ + - - ++ + } N 1 + + + - Os+ Os+ Os+ Peroxidase uses H 2 O 2 and consumes electrons at E 2 Glucose oxidase produces H 2 O 2 Glucose oxidase produces electrons at E 1 e- • P. Pescador DEA thesis (2004) Biofuel cells

Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE SENIOR MEMBERS AND PROJECT MANAGERS • Magda Constanti (Lector, Microbiology) • Teresa Hechevarria (Torres Quevedo, Chemical Engineering) • Ioanis Katakis (Associate professor, Chemical Engineering) • Petros Lenas (Ramon y Cajal, Tissue Engineering) • Przemyslaw Los (Visiting Professor, Physical Chemistry) • Josep Ma Montornés (Post doctoral fellow, Org. Synthesis) • Susana Rodríguez (Ramon y Cajal, Bioprocess Engineering) • Jose Luis Toca-Herrera (Ramon y Cajal, Nanotechnology)

Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE PERMANENT TECHNICIANS AND SUPPORT • Barbara Vastenavond (Administration) • Lea Gragert (Administration and accounting) • Mònica Mir (Technician, Electrochemistry) • Joaquim Solana (Marketing, Project support) • Diego Bejarano (Technician, Chemical & Process Engineering) • Marta Jimenez (Technician, microbiology) • Mireia. Massanell (Technician, Immunology) • Cristina Sanz (Technician, Analytical techniques) • Xavier Roch (IT Technician)

Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE GRADUATE STUDENTS AND TRAINEES • Mònica Mir (Ph. D Student, Bioelectrochemistry) • Srujan Dondapati (Ph. D Student, Bioelectrochemistry) • Paula Pescador (Ph. D Student, Molecular biology/fuel cells) • Veronica Saravia (Ph. D Student, Tissue engineering) • Viviana Duarte (Ph. D Student, Immunosensors) • Frank Jeyson (Ph. D Student, Biofuel cells) • Kelly Briceño (Ph. D Student, Bioelectrochemistry) • Peter Pfeiffer (Ph. D Student, Biomolecular interactions) • Rory Mc. Nally (MS trainee, Microbiology) • Denise Hogan (MS trainee, Microbiology) • Antoni Montseny (Undergraduate trainee) • David Romero (Undergraduate trainee) • Josep Pamies (Undergraduate trainee)

Applied Biotechnology Innovation Centre OUR PRODUCTIVITY IN PERSPECTIVE • Ph. D Theses: • DEA Theses: • Publications/PDI/year: • Citations/PDI/year: DATA BBG 2, 0 9, 0 2, 5 21, 0 (1995 -2004) (ISI 1990 -1999) DATA Catalunya (all areas) • Citations/article/year: 1, 7 (1994 -1998) DATA USA (Chemical Engineering source: NRC 1990 -94) • Citations/PDI/year: 23, 44 (rank #1 U of Minnesota) 20, 78 (rank #2 Stanford U) • Publications/PDI/year: 4, 6 (rank #1 U of Minnesota) 2, 5 (rank #13 SUNY, Buffalo) 1, 7 (rank #72 U of Tulsa)

Applied Biotechnology Innovation Centre ACKNOWLEDGEMENTS Our work is financed by: • INNOCUOUS (Immunostrips for TCA detection) • MICROPROTEIN (Patterning and arraying) • HEALTHY AIMS (Fuel cells) • CIDEM and our Clients • URV, DURSI, MECD