AP Biology Lab 6 Cellular Respiration Brainstorm What

AP Biology Lab 6 Cellular Respiration

Brainstorm: What factors can affect the rate of cellular respiration?

Lab Notes Respirometer: used to measure volume of O 2 consumed during respiration As CO 2 is produced, it will be removed by KOH. CO 2 (gas) + 2 KOH K 2 CO 3 (solid) + H 2 O As O 2 is consumed, gas volume in respirometer decreases Control: respirometer with nonliving matter (glass beads) Measure changes in temp. and air pressure Equilibration: assembled respirometers sit in H 2 O for 5 min. before data is collected Data collection: measure O consumed every 5 min.

Respirometers

Equilibration of Respirometers

Materials See lab handout. Supplies available today: Germinating seeds (48 hr germination): sugar snap peas, lima beans, navy beans, lentils, beets Germinating seeds (24 hr germination): sugar snap peas, lentils Non-Germinating seeds: same as above Pillbugs …Your choice!

Inquiry Investigation Lab Group Problem: How does _____ affect cellular respiration? Hypothesis (If…, then… because…) Experimental Design (IV, DV, control, constants etc. ) Materials Procedure (Simple flow chart) Data Table (see next slide)

Sample Data Table Difference (Diff. ) = (initial reading at time 0) – (reading at time X) Corrected Difference (Corr. Diff. ) = (Initial pea reading at time – pea seed reading at time X) – (initial bead reading at time 0 – bead reading at time X)

Analysis & Conclusion Analysis: Graph (O 2 consumed vs. time) Use corrected difference values for O 2 consumed Determine respiration rates (Use lines of best-fit for this lab) Compare class data Conclusion – Explanation Tool