Antibody Structure Function Application Structure Heavy Chain Light
Antibody • Structure • Function • Application
Structure Heavy Chain Light Chain Constant Domain Variable Domain Fab Fc
Function • Antibodies protect us from invaders by labeling foreign substances. • Each B-cell that produces antibodies is specialized to label only one antigen. • A limited number of genes is able to produce a virtual unlimited number of antibodies.
V and J gene segments(p. 990)
Application • • • Monoclonal and polyclonal antibodies ELISA Immunoprecipitation(IP) Western Blotting Immunoflorescence
Polyclonal Antibodies
Monoclonal Antibodies
ELISA ~Enzyme-Linked Immuno. Sorbent Assay ~ • Biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample http: //gmo. doh. gov. tw/Web/images/flash/pcr 2. swf
Immunoprecipitation(IP)
Western Blotting Protein first separated by size in a thin gel transferred to support membrane probed with solutions of antibodies that specifically bindd can be visualized by color products
Immunofluorescence is the labeling of antibody or antibody with fluorescent dyes. This technique is often used to visualize subcellular distribution of biomolecules of interest.
Antibody的真面目
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