Animal Science 434 Lecture 16 Gamete Transport Sperm
- Slides: 33
Animal Science 434 Lecture 16: Gamete Transport
Sperm Transport in the Male Tract Fluid Flow Cilia Fluid Flow Muscle Contractions
Sperm Transport in the Male Tract Acquisition of Fertilizing Ability and Motility • • No protein synthetic ability addition or loss of plasma membrane proteins and lipids redistribution of proteins and lipids within sperm changes in lipid diffusion coefficients
Sperm Transport in the Male Tract Addition of Decapacitation Factor • protein, glycolipid and/or lipid • stabilizes the plasma membrane Addition of DF From Accessory Gland Secretions
Site of Ejaculation Site of ejaculation Semen Characteristics Species Vagina slight coagulation of ejaculate human, rabbit vagina semen with high sperm concentration cattle, sheep uterus voluminous, distention of cervix horse voluminous, retention of penis during copulation dog, pig vagina plug rodents cervix, uterus
Sperm Loss From Female Tract • retrograde flow • phagocytosis by neutrophils
Experiment on Retrograde Flow in the Cow Uterine Horn Insemination at 3 different spots Uterine Body Cervix
From the Vagina Cumulative %% Sperm Recovered Cumulative From Sperm Vagina 20 15 0 Uterine Body 10 Uterine Horn 5 0 1 2 3 4 5 6 7 Hour After Insemination 8
cervix Cumulative % Sperm Recovered From the Vagina 60 Cervix 50 40 Uterine Horn 30 20 10 0 0 1 2 3 4 5 6 Hour After Insemination Hours After Insemination 7 8
Assume 10 million inseminated Uterine Horn at 8 hr = 25% loss or 75% retained = 7. 5 million sperm retained Cervix at 8 hr = 60% loss or 40% retained = 4 million sperm retained
Evolutionary Strategies for Dealing With Retrograde Flow of Semen • coagulation of semen or a plug • concentrated semen • semen deposited in uterus
Sperm Loss From Female Tract • retrograde flow • phagocytosis by neutrophils
Neutrophile Infiltration of Uterus
Phases of Sperm Transport • rapid » 15 -30 min • sustained » 6 - 12 hr • oviductal » at ovulation
Evidence for Sustained Transport Ligatures at Utero-tubal Junction
Effect of Oviduct Transection on Fertilization in Heifers Interval from mating* to transection of UTJ Oocytes Fertilized 6 0% 8 50% 12 83% * Mating was done at the start of estrus.
Percentage of Oocytes Fertilized Sperm Transport in the Pig }
Ovulation Oocyte Cumulus Cells Follicular Fluid
Cue for ovulation may effect isthmus to trigger oviductal transport
Location of Fertilizing Sperm 6 -12 hr 18 -24 hr 1 -2 hr
Barriers To Sperm Transport
Cervix
Barriers To Sperm Transport Uterotubal Junction
Ovulation Oocyte Cumulus Cells Follicular Fluid
Factors Regulating Oocyte Transport in the Oviduct Fluid Flow Remains closed until day 3 - 5 post ovulation
Total Fluid Production Toward Ampulla Toward Uterus
Factors Regulating Oocyte Transport in the Oviduct Site of Fertilization No Contractions Cilia Muscle Contractions Fluid Flow Remains closed until day 3 - 5 post ovulation
Now view the video podcast entitled View Video on Ovulation and Oocyte Transport Found at: http: //www. ansci. wisc. edu/jjp 1/ansci_repro/lec_16_gam ete_tran/ovulation_oocyte_trans. html
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