Animal Biotechnology Herman the transgenic bull with five

Animal Biotechnology

Herman the transgenic bull with five daughters. Natuurinformatie, 2004.

The gene is injected into a fertilised cattle egg 1 Human cell DNA is isolated from the nucleus of the human cell. The lactoferrin gene is copied 2 A calf possessing a human gene is born 3

The result: BIG ANIMALS

1

Microinjection into fertilized oocyte ﺃﺠﺴﺎﻡ ﻗﻄﺒﻴﺔ Polar bodies Nucleolus Suction Holding ﺗﺜﺒﻴﺖ capillary ﻣﺎﺻﺔ Injection capillary ﺍﻟﻤﺮﺍﺩ ﻧﻘﻠﻪ ﺍﻟﺠﻴﻦ Male pronucleus ﺍﻻﻭﻟﻴﺔ ﻧﻮﺍﺓ ﺍﻟﺬﻛﺮ Female ﺍﻻﻭﻟﻴﺔ pronucleus ﻧﻮﺍﺓ ﺍﻷﻨﺜﻰ Zona ﺟﺎﻫﺰﺓ pellucida ﻟﻠﺤﻘﻦ ﺑﻮﻳﻀﺔ ﻣﺨﺼﺒﻪ Successful microinjection causes swelling of the pronucleus The damage triggers DNA repair system during which the transgene integrates

Microinjection into zygotes of different species pig, cattle (180 m) mouse (85 m), rat (100 m)

Tail tip/ear punch Tg- Tg+ Proteinase K PCR DNA extraction Restriction and electrophoresis MW M H Tg- Tg+ M, mouse H, human Hind III Analysis of transgenecity

embryonic stem cells Fertilized oocyte Cultured ES cells ﺍﻟﺒﻮﻳﻀﺔ ﺍﻟﻤﺨﺼﺒﺔ Selection & cell transfer DNA transfection (electroporation) ﺍﻟﺼﻌﻖ ﺍﻟﻜﻬﺮﺑﺎﺋﻲ Inner cell mass (pluripotent) Blastocyst