Angelica Sinensis Polysaccharide Suppresses EpithelialMesenchymal Transition and Pulmonary

  • Slides: 1
Download presentation
Angelica Sinensis Polysaccharide Suppresses Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via a DANCR/AUF-1/FOXO 3 Regulatory

Angelica Sinensis Polysaccharide Suppresses Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via a DANCR/AUF-1/FOXO 3 Regulatory Axis Weibin Qian, Xinrui Cai, Qiuhai Qian, Dongli Wang, Lei Zhang 1 Department of Lung Disease, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, China. 2 Department of Traditional Chinese Medicine, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University &#x 00026; Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China. 3 Department of Endocrinology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, China. 4 Department of Personnel Section, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, China. 5 Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, China Figure 5. DANCR promotes translation of FOXO 3 gene by binding to AUF 1. (A) RIP was performed using anti. AUF 1 and control Ig. G antibodies, followed by q. RT-PCR to determine the enrichment of DANCR and U 6 was used as the negative control, &#x 0002 A; P&#x 0003 C; 0. 01. (B) Experimental validation of AUF 1 knockdown in RLE-6 TN cells by q. RT-PCR and western blotting, &#x 0002 A; Aging and Disease, 2020, 11(1), 17 -30. DOI: 10. 14336/AD. 2019. 0512 P&#x 0003 C; 0. 001. (C) m. RNA and protein levels of FOXO 3 in RLE-6 TN cells silenced with AUF 1. (D) Western blotting was performed to detect the protein expression levels of FOXO 3 in RLE-6 TN cells overexpressing DANCR or (and) silenced with AUF 1. (E) RLE-6 TN lysates were incubated with in vitro -synthesized, biotin-labeled control Lac. Z DNA probes or DNA probes against DANCR for the biotinylated oligonucleotide pulldown assay. &#x 0002 A; P&#x 0003 C; 0. 01 compared to respective Lac. Z probes. (F) Endogenous AUF 1 binding to FOXO 3 m. RNA was modified by DANCR overexpression, &#x 0002 A; P&#x 0003 C; 0. 01. (G) Control plasmids or DANCR plasmids transfected RLE-6 TN cells were cultured with 20 &#x 003 BC; g/ml cycloheximide