Analyticon Biotechnologies AG Lichtenfels Germany agile affordable accurate

Analyticon Biotechnologies AG Lichtenfels - Germany agile - affordable - accurate 1

Basics of Hematology Hemolyzer ® agile - affordable - accurate 2

Measuring Principles § Laser light scatter technology for 5 -part WBC differential § Impedance measurement for WBC, RBC und PLT § Light absorbance for measuring HGB agile - affordable - accurate 3

Types of blood cells § RBC: 95% of all blood cells § PLT: ~4. 8% of all blood cells § WBC: ~0. 2% of all blood cells § § § NEU: 40 -75% LYM: 20 -45% MON: 2 -8% EOS: 1 -6% BAS: 0 -1% agile - affordable - accurate 4

Differential Lysing Process PLT & RBCs destroyed LYM MON GRAN WBCs selectively shrank to nuclei agile - affordable - accurate 5

3 -part Differential WBC Histogram PLT & RBC LYM MON GRAN & HGB agile - affordable - accurate 6

4 -part Differential WBC Scattergram Neutrophiles Monocytes Size RBC GHOST § Basophiles cannot be reliably differentiated from the rest of the white blood cells § Additional optical count is needed Complexity High angle forward scatter Eosinophiles Lymphocytes Low angle forward scatter agile - affordable - accurate 7

Hemolyzer® 3 agile - affordable - accurate 8

Hemolyzer® 3 § Provides 20 parameters CBC including 3 -part WBC differential § 3 Histograms: WBC, RBC, PLT § Throughput: 60 samples per hour § 6 QC lots, Levey-Jennings diagrams § Database capacity: 10, 000 § Only 3 reagents (cyanide-free) § Automatic monitoring of reagent status agile - affordable - accurate 9

Hemolyzer® 3 § Sample Volume: 25 μl (open tube sampling) § Automatic clogging prevention § Large, high resolution colour LCD § Easy, intuitive operation § Interfaces: RS 232, USB § Printout via internal thermal printer (optional also via external printer) § Compact design: § 350 x 490 x 385 mm § Weight: 15 kg agile - affordable - accurate 10

Hemolyzer® 3: Maintenance § Daily Maintenance Hemolyzer Hypocleaner is inserted as sample and “measured” agile - affordable - accurate 11

Hemolyzer® 3: Key Benefits § Safe Operation § The sample is being aspirated inside the analyzer, practically excluding the risk of injuries for the user § High speed § Results within 60 seconds § Reliable Instrument § Easy maintenance by the user agile - affordable - accurate 12

Hemolyzer® 3: Competitors Model Distributor Hemolyzer® 3 Analyticon Cell-dyn® 1800 Abbott AC • T Diff Beckman Coulter AC • T Diff 2 Beckman Coulter ABX Micros 60 Horiba WBC diff. Number of parameters Histograms Throughput (PT/h) Sample volume [μl] 3 20 WBC, RBC, PLT 60 25 3 18 WBC, RBC, PLT 60 30 3 18 WBC, RBC, PLT 50 12 3 18 WBC, RBC, PLT 50 18 3 18 WBC, RBC, PLT 60 (open); 55(closed) 10 /- 3 optional - - - /- 10, 000 Levey-Jennings 66 x 44 x 52 55 18 -30°C /- 3 /- 250 Levey-Jennings, IQAP 38 x 42 x 39 16 / 3 /- 250 Levey-Jennings 45 x 49 x 40 20 / 3 optional - - - /- 1; 78 (with smart card) 36 x 42 x 32 14 16 -34°C Open/Closed sampling /- STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) - Colour display Touch Screen - Internal printer RS-232/USB / Database capacity 10, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 35 x 49 x 38. 5 Weight [kg] 15 Operating temperature 15 -35°C agile - affordable - accurate 13

Hemolyzer® 3: Competitors Model Distributor Hemolyzer® 3 Analyticon M 16 M/C/20 M/C Medonic M 16 S/20 S Medonic CA 620 Medonic KX 21 Sysmex WBC diff. Number of parameters Histograms Throughput (PT/h) Sample volume [μl] 3 20 WBC, RBC, PLT 60 25 3 16/20 WBC, RBC, PLT 65 100 (open); 250 (closed) / (C only) 3 / Levey-Jennings 29 x 41 x 46 18 18 -32°C 3 16/20 WBC, RBC, PLT 65 300 3 9/16/20 WBC, RBC, PLT 60 125 3 18 WBC, RBC, PLT 60 50 / 3 (2 x 20) / Levey-Jennings 33 x 46 22 18 -32°C /optional 3 optional - - / 220 Levey-Jennings 42 x 35 x 46 22 18 -32°C /- 2 optional - - / 300 Levey-Jennings 42 x 48 x 36 28 Open/Closed sampling /- STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) - Colour display Touch Screen - Internal printer RS-232/USB / Database capacity 10, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 35 x 49 x 38. 5 Weight [kg] 15 Operating temperature 15 -35°C agile - affordable - accurate 14

Hemolyzer® 3: Competitors Model Distributor Hemolyzer® 3 Analyticon BC 3000+ Mindray BC 3200 Mindray BC 3600 Mindray WBC diff. Number of parameters Histograms Throughput (PT/h) Sample volume [μl] 3 20 WBC, RBC, PLT 60 25 3 19 WBC, RBC, PLT 60 13 3 21 WBC, RBC, PLT 60 20 /- 3 optional - - / 35000 Levey-Jennings 39 x 46 x 40 21 15 -30°C -/ 5 optional - - / 35000 Levey-Jennings 39 x 46 x 42 25 15 -30°C /optional 3 optional - / 40000 Levey-Jennings 45 x 40 x 45 28 15 -30°C Open/Closed sampling /- STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) - Colour display Touch Screen - Internal printer RS-232/USB / Database capacity 10, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 35 x 49 x 38. 5 Weight [kg] 15 Operating temperature 15 -35°C agile - affordable - accurate 15

Hemolyzer® 3: Competitors Model Hemolyzer® 3 Distributor Analyticon WBC diff. Number of parameters Histograms Throughput (PT/h) Sample volume [μl] 3 20 WBC, RBC, PLT 60 25 Open/Closed sampling /- STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) - Colour display Touch Screen - Internal printer RS-232/USB / Database capacity 10, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 35 x 49 x 38. 5 Weight [kg] 15 Operating temperature 15 -35°C Celltac α MEK 6400/6410/6420 Nihon Kohden Mythic 18 H 18 light Celly Plus Orphee SFRI Biocode Hycel 3 18 WBC, RBC, PLT 63 30 3 18 WBC, RBC, PLT 60 9. 8 3 20 WBC, RBC, PLT 60 9. 6 3 18 WBC, RBC, PLT 60 10 / 4 optional - optional /400 Levey-Jennings / 3 optional - - optional / 1, 500 Levey-Jennings 3 - / 20, 000 Levey-Jennings 3 optional / 1, 000 Levey-Jennings 23 x 45 x 38. 3 25 x 34 x 35 9 33 x 43 x 38 23 18 -35°C 32 x 35. 2 x 36. 8 9. 5 18 -35°C agile - affordable - accurate 16

Hemolyzer® 5 agile - affordable - accurate 17

Hemolyzer® 5 § Provides 24 parameters CBC including 5 -part WBC differential § 2 Histograms: RBC, PLT § 2 Scattergrams: 4 -diff WBC, BASO § Throughput: 60 samples per hour § 6 QC lots, Levey-Jennings diagrams § Database capacity: 100, 000 § Only 3 reagents (cyanide-free) § Automatic monitoring of reagent status agile - affordable - accurate 18

Hemolyzer® 5 § Sample Volume: 100 μl (open/closed, whole blood) § Automatic clogging prevention § Large colour LCD touch screen § Easy, intuitive operation § Autoloader (100 samples) optional § Interfaces: RS 232, USB § Printout via external Microsoft® Windows. TM compatible printers agile - affordable - accurate 19

Hemolyzer® 5: Dimensions § Compact design: 410 x 465 x 515 mm § Weight: 35 kg (with autoloader: 49 kg) agile - affordable - accurate 20

Hemolyzer® 5: Autoloader § Plug-In; can be added any time § Based on linear racks § Up to 100 samples (10 racks) § Cap recognition and cap piercing § Monovette®, Vacutainer®, Vacuette® § Integrated barcode reader § Integrated sample mixer § Using the sample needle agile - affordable - accurate 21

Hemolyzer® 5: 4 -Diff Scattergram Neutrophiles Complexity High angle forward scatter Eosinophiles Monocytes Size RBC GHOST Lymphocytes Low angle forward scatter agile - affordable - accurate 22

Hemolyzer® 5: Basophile Scattergram § in the 4 -part differential the basophiles cannot be reliably differentiated from the rest of the white blood cells § additional optical count is needed for basophiles § in the WBC/HGB dilution all WBC’s are (over)lysed with the exception of the basophiles § after the impedance count the WBC/HGB dilution is transferred to the optical head for BASO count agile - affordable - accurate 23

Hemolyzer® 5: Maintenance § Daily Maintenance Hemolyzer Hypocleaner is inserted as sample and “measured” § Regular Maintenance (depending on throughput) § § Cleaning of measurement head Cleaning of shear valve agile - affordable - accurate 24

Hemolyzer® 5: Key Benefits § Safe Operation § The sample is being aspirated inside the analyzer, practically excluding the risk of injuries for the user § Patented Laser Technology § High speed § Results within 60 seconds § Reliable Instrument § Easy maintenance by the user § Autoloader (100 samples) § Upgrade can be done at any time agile - affordable - accurate 25

Hemolyzer® 5: Competitors Model Hemolyzer® 5 Cell-dyn® 3200 CS Cell-dyn® 3200 AL Cell-dyn® 3700 CS Cell-dyn® 3700 SL Distributor Analyticon Abbott WBC diff. Number of parameters Histograms Scattergrams 5 24 RBC, PLT 4 diff (L-M-N-E), BAS 5 22 RBC, PLT WBC, RBC, PLT, RET 5 23 RBC, PLT WBC, RBC, PLT, Throughput (PT/h) Sample volume [μl] 60 100 71 72 90 90 240 (closed); 150 (open) 240 (closed); 130 (open) 355 (AL); 240 (closed); 130 (open) / / 4 4 (50) (100) / /10, 000 Levey-Jennings 83 x 48 x 59 83 x 48 x 74 76 x 61 x 56 76 x 68 x 79 97 106 86 131 Open/Closed sampling / STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) optional (100) Standalone Touch Screen Internal printer RS-232/USB 1/4 Database capacity 100, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 40 x 45 x 50 Weight [kg] 35 agile - affordable - accurate 26

Hemolyzer® 5: Competitors Model Hemolyzer® 5 AC • TTM 5 Diff OV/CP AC • TTM 5 Diff AL Hm. X ABX Pentra (XL) 80 Distributor Analyticon Beckman Coulter Horiba WBC diff. Number of parameters Histograms Scattergrams 5 24 RBC, PLT 4 diff (L-M-N-E), BAS 5 26 WBC, RBC, PLT 4 diff (L-M-N-E) 5 24 RBC, PLT Throughput (PT/h) Sample volume [μl] 60 100 60 60 80 75 53 (CBC + diff); 30 (CBC) 185 (closed); 125 (open) 53 (CBC + diff); 30 (CBC) Open/Closed sampling / STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) optional (100) Standalone Touch Screen Internal printer RS-232/USB 1/4 Database capacity 100, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 40 x 45 x 50 Weight [kg] 35 / (CP) 5 optional (OV)/-(CP) / 10, 000 IQAP 44. 4 x 58 x 50. 1 36. 2 / 5 (100) / 10, 000 IQAP 81. 5 x 58 x 54 55 / 5 / 5, 000 IQAP 61. 5 x 61. 7 x 84. 8 94. 5 / 5 (100) (laser) /2 10, 000 Levey-Jennings 82 x 57 x 54 cm 55 agile - affordable - accurate 27

Hemolyzer® 5: Competitors Model Hemolyzer® 5 BC 5300 BC 5380 BC 5500 BC 5800 Distributor Analyticon Mindray WBC diff. Number of parameters Histograms Scattergrams 5 24 RBC, PLT 4 diff (L-M-N-E), BAS 5 27 3 1 5 27 RBC, PLT 4 diff (L-M-N-E), BAS 5 29 RBC, PLT 4 diff (L-M-N-E), BAS Throughput (PT/h) Sample volume [μl] 60 100 60 60 80 (AL); 60 (manual) 90 20 (CBC + diff); 15 (CBC) 180 (closed); 120 (open) Open/Closed sampling / STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) optional (100) Standalone Touch Screen Internal printer RS-232/USB 1/4 Database capacity 100, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 40 x 45 x 50 Weight [kg] 35 / 5 optional 40, 000 41 x 53 x 47 45 / 5 (30) 40, 000 / 6 optional (50) /4 40, 000 66. 4 x 61. 3 x 58. 5 73 / 6 optional (50) /5 40, 000 66. 4 x 61. 3 x 58. 6 76 agile - affordable - accurate 28

Hemolyzer® 5: Competitors Model Hemolyzer® 5 XS 800 i XS 1000 i XT 1800 i/2000 i Distributor Analyticon Sysmex WBC diff. Number of parameters Histograms Scattergrams 5 24 RBC, PLT 4 diff (L-M-N-E), BAS 5 24 RBC, PLT 60 20 / 4 optional / 10, 000 Levey-Jennings 32 x 50 x 41 24 60; AL: 52 20 / 4 optional (20) / 10, 000 Levey-Jennings 32 x 40 x 41 24 5 24/31 RBC, PLT 4 diff, WBC/Baso, PLT, Ret (2000 i) 80 85; 150 (AL) / 5 optional 10, 000 Levey-Jennings 53 x 63 x 50 52 Throughput (PT/h) 60 Sample volume [μl] 100 Open/Closed sampling / STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) optional (100) Standalone Touch Screen Internal printer RS-232/USB 1/4 Database capacity 100, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 40 x 45 x 50 Weight [kg] 35 agile - affordable - accurate 29

Hemolyzer® 5: Competitors Model Hemolyzer® 5 Celltac E MEK-7222 Celltac E MEK-8222 Mythic 22 OT/CT Mythic 22 AL Distributor Analyticon Nihon Kohden Orphee WBC diff. Number of parameters Histograms Scattergrams 5 24 RBC, PLT 4 diff (L-M-N-E), BAS 5 22 RBC, PLT 5 -diff Throughput (PT/h) 60 51 80 50(OT)/45(CT) Sample volume [μl] 100 55 (CBC + diff); 30(CBC) 56 (CBC + diff); 30 (CBC) 17 (OT)/20 (CT) 20 /optional 5 optional / Levey-Jennings /optional 5 optional (50) optional / 100, 000 Levey-Jennings / (CT only) 3 optional 1/2 1, 500 Levey-Jennings / 3 (50) 1/5 1, 500 Levey-Jennings 61. 3 x 58. 3 x 55 55 25 x 34 x 35 11 48 x 40 25 38. 2 x 48. 5 x 52 38 Open/Closed sampling / STAT Function Number of reagents 3 Bar code reader optional Autoloader (capacity) optional (100) Standalone Touch Screen Internal printer RS-232/USB 1/4 Database capacity 100, 000 QC Levey-Jennings Dimensions Size (Wx. Dx. H) [cm] 40 x 45 x 50 Weight [kg] 35 agile - affordable - accurate 30

agile - affordable - accurate 31