An Introduction MICROSCOPY Microscopy 1 b Given the
An Introduction MICROSCOPY
Microscopy 1 b. Given the appropriate supplies, and equipment, apply the basic principles of microscopy to properly focus a microscope with no more than three instructor assists. (1) Components of a Compound Microscope (2) Types of Microscopes (3) Care of the Microscope (4) Operation (5) ORM Group Discussion
Oculars Two that magnify the image 5 x or 10 x May contain ocular micrometer Microscopic ruler used to measure objects Calibrated using a stage micrometer etched into a glass slide
Objectives Main magnifiers that capture primary image Located on spring mounted revolving nosepiece Named depending on power and function
Objectives Scanning 4 x/5 x power Not used in hemotology
Objectives Low power 10 x power For initial focusing and some cell counts
Objectives High dry 40 x to 45 x power Used for some cell counts
Objectives Oil immersion 100 x power (may have a black ring) Special immersion oil is used between slide and objective Better resolving power at higher magnification Used with fixed and stained slides
Body Contains lens system Oculars at top Objectives at bottom Interpupillary distance control Adjusts distance between oculars Allows viewer to see one field of view
Arm Connects the base to body Holds stage and its components Used to carry the microscope Houses the coarse and fine focus knobs Coarse – used to locate initial image Fine – used to obtain optimum resolution
Stage Movable platform used to hold slide and bring item of interest into field of view Two control knobs Side-to-side movement Front-to-back movement Stage clips hold slide in place Light condenser attached to the underside of the stage
Base Contains the transformer which converts electrical power from AC to DC to provide power to illuminator Houses illuminator (light source) Control switch is located on the base On/off style Variable intensity
Condenser Converges (bends) light into a beam by use of a convex lens Condenser control knob moves it up and down
Iris diaphragm Attached to underside of condenser Multi-leafed circular opening used to control the amount of light transmitted to condenser Adjusted by lever or dial on condenser
Types of microscopes Phase Contrast Epifluorescence Electron Darkfield Polarized
Care of Microscope Storage Dust and lint Oculars and objectives Mirror/Illuminator/Condenser
Operation Plug in and turn on Lower stage to lowest position Place slide into stage clip Place on low power objective (10 x) Open iris diaphragm half-way Place condenser in lowest position Focus using coarse focus knob Adjust light and fine focus Adjust interpupillary distance
Operation Rotate nosepiece until high dry objective clicks in to place and focus using fine focus Switch between high dry and oil immersion objective Place immersion oil on slide Rotate the nosepiece until oil immersion objective clicks into place Use fine focus knob to adjust to optimum resolution
Microscopy 1 b. Given the appropriate supplies, and equipment, apply the basic principles of microscopy to properly focus a microscope with no more than three instructor assists. (1) Components of a Compound Microscope (2) Types of Microscopes (3) Care of the Microscope (4) Operation (5) ORM Group Discussion
- Slides: 19