An example of Flow Cytometric DNA Analysis as
- Slides: 14
An example of Flow Cytometric DNA Analysis as a diagnostic tool J. Chezar Western Galilee Hospital-Nahariay Israel
History: A 49 year old male referred to the hematology department because of pancytopenia. After 2 dry taps, a bone marrow aspirate resulting in barely 200 u. L of material was obtained and sent for flow cytometry. The sample was stained with the following antibody combinations. CD 45 PC 7/CD 34 ECD/CD 33 PC 5/ CD 117 PE/ HLA-DR FITC, CD 45 PC 7/CD 3 ECD/CD 56 PC 5/CD 8 PE/CD 4 FITC+CD 19 FITC, CD 45 PC 5/CD 13 PE/CD 16 FITC, CD 45 PC 5/CD 64 PE/CD 14 FITC. DNA cell cycle analysis was performed alone and in combination with CD 45 FITC and glycophorin A FITC, CD 13 FITC. In addition a bone marrow biopsy was sent to pathology. Immunohistochemistry was done with peroxidase conjugated LCA, MPO, CD 34, Td. T, CD 15, CD 117, PAS, CD 43, CD 138, CD 31, CD 79 a, Factor VIII, Ker, CD 68, CD 3, B and T cell antibodies
BLASTS
Aberrant population Normal Bone Marrow Patient Bone Marrow
Myeloid phenotype = Blasts?
MPO: GRAN+ / BLASTS -
PAS NEG IN BLASTS
Normal 2 n Aneuploid (A)
To verify identity of aberrant population – double stain with PI for DNA and relevant FITC labeled antibody
A Aneuploid population NOT leukocytic
A Aneuploid population NOT thrombocytic
Glycophorin A + A Aneuploid population IS erythroid !!
Glycophorin
Erythro Leukemia DNA analysis and subsequent double staining with appropriate antibodies lead to the correct diagnosis.
- Replication
- Bioflix activity dna replication lagging strand synthesis
- Coding dna and non coding dna
- Replication process
- Chapter 11 dna and genes
- Absorptive atelectasis
- Trach collar oxygen flow rates
- Simple mask
- Difference between laminar and turbulent flow
- Internal and external flow
- Flow of energy vs flow of matter
- Oikos meaning
- Transform flow and transaction flow in software engineering
- Transform flow and transaction flow
- Definition