AN AUTOPERFUSING UMBILICAL CORD BLOOD COLLECTION INSTRUMENT Contents

AN AUTO-PERFUSING UMBILICAL CORD BLOOD COLLECTION INSTRUMENT

Ø Contents �Introduction �Why this advancement in the field of ‘Biomedical Instrumentation ‘? �UCB Collection Instrument �Systems � Two in UCB collection Instrument Fraction UCB collection �Applications �Conclusion

Introduction Ø �An Automated Blood collection Instrument comprises of Mechanical , electronic and Control components. �UCB from the placenta provides a rich source of highly proliferative cells for many clinical uses as it contains rich Hematopoietic Stem Cells (HSCs). �Current collection of UCB uses a syringe to extract blood from placenta. � An Automated UCB collection instrument to

Why this advancement in the field of ‘Biomedical As an ex-utero approach, the challenges to Instrumentation be addressed during the‘? collection process will include: (1) Limited time window for further collection. (2) Need to stay robust of cross contamination from maternal blood. (3) Replication of a uterus compression force on the placenta. (4) In Ex-Utero, infusion of saline back into the placenta will dislodge the cells draining

Ex-Utero

Ø UCB Collection Instrument Functionally, it comprises the following systems: • Placenta handling system. • Controlled air chamber. • Auto-perfusion system. • Time window widening system. • Open-architecture control system.

Ø Placenta Handling System Main parts of the system : • Air tight lid • Placenta Bowl • Supporting Legs • Base

Ø Parts Of The System

Ø Controlled Air Chamber The lid houses the interfaces to three standard ports which are connected to • Proportional Valve • Solenoid valve • An Analog Pressure Sensor

• The air pressure variation in the chamber will be converted to a patterned force profile acting on the maternal surface of the placenta via a pressure membrane as shown in Fig.

Finite Element Analysis(FEA)

Ø Auto-Perfusing System • Enables perfusion automatically. • Consist of catheter and three valves. • A catheter is inserted into a vein on the umbilical cord. • Other end of the catheter a three-way valve is connected.

Ø Time -Widening Window System �A two-pronged approach is adopted. �A vibrator, rendering a low frequency vibration of about 2 Hz. �Secondly, the exposed end of the cord can be rapidly cooled to a low temperature once it is cut.

Ø Open –Architecture Control System �Needed for all components to work together as one unified instrument. �A standalone embedded control system is employed based on the National Instrument (NI) single board solution sb. RIO-9632.

The I/O interface from the board to the other components is depicted in fig below :

Ø Two-fraction UCB collection �Synchronous operations with current procedures of code blood banks along with autoperfusion is also possible.

The flowchart of the overall automated procedure is shown in fig

Ø Conclusion �An automated and self-perfusing instrument enables the more efficient and extensive harvesting of HCS. �Can be used for the pediatric and adult treatment. �Instrument maximizes the explusion of cord blood. �a stand-alone system to retrieve cord blood in a one stage collection

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