Amanda Tulk Linda Alfred CIX Cabinet Irradiators Systems

Amanda Tulk Linda Alfred CIX Cabinet Irradiators Systems Xenx & SARRP Image Guided Systems

Xstrahl Range for In Vitro to In Vivo CIX 320 k. V CIXD HVL 3 mm Cu HVL 2 mm Cu Xenx and SARRP

CIX Filters – HVL and Dose Rates l l l HVL’s can be provided as ~2. 0 mm Cu, 3. 7 mm Cu at 300 k. V 10 m. A Custom made HVL’s and energies can optionally be commissioned Field size at 50 cm FSD is 30 cm diameter Homogeneity

In Vivo : 137 Cs compared to X-ray 300 k. V Conclusions of Studies In vitro: radiation toxicity to cell culture is similar for both radiation types in common cancer cell lines l l X-ray RBE of HBEC cells of 1. 5 -1. 6, HVL 1. 0 mm Cu l Gamma dose: 470, 940, 1880 m. Gy; l X-ray dose: 360, 720, 1440 m. Gy X-ray RBE of He. La cells of 1. 2 and 1. 5 for A 549, HVL 3. 7 mm Cu l Gamma dose: 470, 940, 1880 m. Gy; l X-ray dose: 720, 1440, 2890 m. Gy Report finding stated : Xray sources can be used successfully in studies of radiation cytotoxicity in culture as in the case of 137 Cs sources Scott et al. Radiation Protection Dosimetry, 2012 (in vitro) Potter et al. Sandia Report, 2013

CIX In Vivo : 137 Cs V X-Rays Depth Dose Percentage depth dose (%) Depth Dose Distribution HVL = 3 mm Cu Field: 20 x 20 cm² Percentage depth dose in water, 6 x 6 cm² field 137 Cs Technical Report Series 328, Planning of radiotherapy facilities, Report of a joint IAEA/WHO meeting, World Health Organization, Geneva (1966) Field: 10 x 10 cm² Physical approach to depth dose distributions in a water phantom irradiated by a teleisotope photon beam, Ahuja et al, Med. Phys. 7, 120 (1980)

In Vivo and In Vitro : CIX 3 or CIXD? CIX 3 CIXD

137 Cs compared to X-ray, the effect of BM ablation and reconstitution for in vivo analysis 137 Cs: Gamma emitting, high energy photon X-ray: Electromagnetic energy, low energy photon l 100% mortality achieved at 900 and 1100 c. Gy l Hematologic recovery measured at 30 d and 90 d l No sig diff in peripheral engraftment at doses between 900 -1050 c. Gy l Sig increase in peripheral engraftment at 1100 c. Gy l Sig decrease in peripheral engraftment at 1100 c. Gy l Sig increase in engraftment of B-cells at all doses l Sig decrease in engraftment of B-cells at all doses l l Sign decrease in engraftment of myeloid cells at all doses No difference in T-cells at 900 -950 c. Gy, sig less at 1000 -1100 c. Gy Gamma l l Sign increase in engraftment of myeloid cells at all doses No difference in T-cells at 900 -950 c. Gy, sig more at 1000 -1100 c. Gy 160 k. Vp Gibson et al. Comparative Medicine, 2015.

Bone marrow and cell subtype reconstitution after WBI with either 137 Cs or X-ray Peripheral Myeloid 160 k. Vp or Gamma B-Cells T-Cells Gibson et al. Comparative Medicine, 2015.

Innovate UK Grant Developing Non-animal Technologies Development of a pre-clinical dosimetry service with multipurpose small animal phantom for radiation pre clinical studies

Accurate Pre Clinical Dosimetry is Key CIX 300 k. V CIXD HVL 3 mm Cu HVL 2 mm Cu Xenx and SARRP