All Rights Reserved AEIC 2004 All Rights Reserved

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

3. 0 y = - 0. 15672 x + 1. 3795 x + 0. 0656 R = 0. 9999 2 2. 5 2. 0 OD 0. 1% LOD 1. 5 1. 0 0. 5 0. 0 0. 5 1. 0 1. 5 2. 0 2. 5 %GMO Antibody detects toasted soymeal Antibody not selective for toasted soymeal Soymeal was toasted for 60 min. at 100 ºC All Rights Reserved AEIC 2004

3. 0 y = - 0. 15672 x + 1. 3795 x + 0. 0656 R = 0. 9999 2 2. 5 2. 0 OD 0. 1% LOD 1. 5 1. 0 0. 5 0. 0 0. 5 1. 0 1. 5 2. 0 2. 5 %GMO Antibody detects toasted soymeal Antibody not selective for toasted soymeal Soymeal was toasted for 60 min. at 100 ºC All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

Trait CP 4 EPSPS Cry 1 Ab Cry 9 C Cry 2 A Cry 3 A NK 603 Agdia E, S Enviro. Logix Neogen E, S E 3, S E, S SDI S E, S E 1, S E 2, S E 3, S E www. agdia. com S S S www. envirologix. com www. neogen. com www. sdix. com E ELISA S strip ® 1 validated by JRC ring test for Roundup Ready Soy bean ® 2 validated by AACC ring test for Yield. Gard (Mon 810) Corn 3 official method AACC international collaborative trial All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

PCR Design Requirements Assay time Sample cost Availability Validation Application ELISA Strips DNA/Line specific Protein specific Sophisticated Moderate Easy 3 -14 days 2 -7 days 5 -10 min $400 -600 $100+ $7 -10 Published/Testing Co Diagnostic Co. Protein specific Diagnostic Co. JRC (qualitative) JRC/USDA Qualitative/semi-quant Qual/semi/quant USDA Qual/ test/ compliance with threshold Limitations Basic p. H, heat, extraction, Same as ELISA false pos, false neg reference materials pro-zone, dynamic sys Protein dependent 1 sample/test Sensitivity ~>0. 1% GMO ~>0. 3% GMO ~1% GMO All Rights Reserved AEIC 2004