All Rights Reserved AEIC 2004 All Rights Reserved

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All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

Step 1 Step 2 Sample plant tissue Weigh Sample Aliquots Step 3 Extract Samples

Step 1 Step 2 Sample plant tissue Weigh Sample Aliquots Step 3 Extract Samples Step 6 Data analysis Step 4 Step 5 ELISA Analysis Aliquot extracts for analysis and sample back-ups All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

All Rights Reserved AEIC 2004

PCR Design Requirements Assay time Sample cost Availability Validation Application ELISA Strips DNA/Line specific

PCR Design Requirements Assay time Sample cost Availability Validation Application ELISA Strips DNA/Line specific Protein specific Sophisticated Moderate Easy 3 -14 days 2 -7 days 5 -10 min $400 -600 $100+ $7 -10 Published/Testing Co Diagnostic Co. Protein specific Diagnostic Co. JRC (qualitative) JRC/USDA Qualitative/semi-quant Qual/semi/quant USDA Qual/ test/ compliance with threshold Limitations Basic p. H, heat, extraction, Same as ELISA false pos, false neg reference materials pro-zone, dynamic sys Protein dependent 1 sample/test Sensitivity ~>0. 1% GMO ~>0. 3% GMO ~1% GMO All Rights Reserved AEIC 2004