Agricultural Institute of Slovenia KIS Lettuce colection Collected

Agricultural Institute of Slovenia, KIS: Lettuce colection Collected in the period from 1988 to 2008. Lactuca sativa - 202 accessions: 175 collected in Slovenia - L. sativa var. capitata: 164, - L. sativa var. longifolia: 9, - L. sativa var. crispa: 3, - L. sativa var. longifolia + capitata: 9. Wild lettuce: 23 accessions: 4 collected in Slovenia - L. seriola: 5, - L. saligna: 18.

Characterization 34 morphological and phenological parameters were evaluated according to the UPOV descriptors for lettuce(TG 13/8) (Guidelines for the conduct of tests for distinctness, uniformity and stability).

Regeneration Acc (g) LAC 1 LAC 2 LAC 3 LAC 4 LAC 5 LAC 6. 37, 7 23, 9 42, 1 6, 5 LAC 7 LAC 8 LAC 9 LAC 10 LAC 11 LAC 12 LAC 13 LAC 14 LAC 15 LAC 17 LAC 18 LAC 19 LAC 20 LAC 21 bb LAC 21 c LAC 22 LAC 23 LAC 24 LAC 25 LAC 28 LAC 29 LAC 30 LAC 31 LAC 32 LAC 33 LAC 34 LAC 35 LAC 38 LAC 39 LAC 42 LAC 45 7, 3 10, 7 4, 4 5, 2 2, 8 0, 6 27, 1 21, 4 7, 3 6, 2 11 2, 8 3, 6 11 9, 5 0, 3 1, 3 3, 9 12 3, 4 18 8 3, 1 1 14 4 1 2 3, 6 1 3, 7 0, 5 LAC 47 43 semen Acc (g) Acc LAC 49 3 LAC 50 LAC 51 LAC 53 LAC 54 LAC 55 LAC 56 LAC 57 LAC 58 LAC 59 LAC 60 LAC 61 LAC 62 LAC 64 LAC 65 LAC 66 LAC 68 LAC 72 LAC 73 LAC 74 LAC 75 LAC 76 LAC 77 LAC 78 LAC 79 LAC 80 LAC 81 LAC 82 LAC 83 LAC 84 LAC 87čs LAC 87 bs LAC 88 LAC 89 LAC 90 LAC 91 LAC 94 LAC 96 0, 7 8 8 27 3, 6 3, 8 1 1 2, 5 8 10 32 36 73 2, 6 27 23 10 50 1, 5 43 2 21 32 22 7, 2 84 36 LAC 97 LAC 99 LAC 103 LAC 104 LAC 106 LAC 107 LAC 108 LAC 112 LAC 113 LAC 114 LAC 118 LAC 119 LAC 123 LAC 124 LAC 127 LAC 128 LAC 129 LAC 131 LAC 134 LAC 135 LAC 139 LAC 140 LAC 142 LAC 146 LAC 147 LAC 148 LAC 150 LAC 151 LAC 152 LAC 155 LAC 157 LAC 160 LAC 165 40 10 15 33 4 31 LAC 168 (g) 12 11 70 13 53 11 90 16 50 38 12 40 22 42 30 9 18 15 2 7 7 4 3 6 55 67 12 200 280 93 6 22 1

Evaluation

Meloidogyne ethiopica RESISTANCE ASSESMENT

RESISTANCE TEST PROCEDURE ggs e 0 300 plant / Estimation of nematode reproduction after 45 dys Estimation of the plant resistance R = resistant (GI < 2, Pf < 1) HR = Hipersensitive reaction (GI > 2, Pf < 1) S = Susceptible (GI > 2, Pf > 1) gall index / nematode population

GALL INDEX LOW INFESTATION HIGH INFESTATION

Results Lettuce accession Rf GBK 10. 42 GB 17 3. 06 GB 61 1. 70 GB 78 2. 22 VANITY 1. 46 LEDA 5. 10 GB 9 1. 04 Table 1: Reproduction factors of M. ethiopica for tested lettuce accessions. Figure 1: Reproduction factors of M. ethiopica for tested lettuce accessions. Estimation of the reproduction of root-knot nematodes: Assessment of the number of eggs and second stage juveniles – final population (Pf): roots were placed in 100 ml H 2 O and cut with a blender. Suspension was poured over sieves (eggs were collected on 30µm sieve) while second stage juveniles were extracted from whole substrate content. Extracted eggs and juveniles were counted using dissecting microscope (Pf). Reproduction factor (Rf) was determined Rf = Pf / Pi.