AgingInduced BrainDerived Neurotrophic Factor in Adipocyte Progenitors Contributes

Aging-Induced Brain-Derived Neurotrophic Factor in Adipocyte Progenitors Contributes to Adipose Tissue Dysfunction Hyun-Doo Song, Sang Nam Kim, Abhirup Saha, Sang-Yeop Ahn, Seun Akindehin, Yeonho Son, Yoon Keun Cho, Min. Su Kim, Ji-Hyun Park, Young-Suk Jung, Yun-Hee Lee 1 College of Pharmacy, Yonsei University, Incheon, Republic of Korea. 2 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea. 3 College of Pharmacy, Pusan National University, Busan, Republic of Korea. Figure 5. pro. BDNF treatment-induced apoptosis/necroptosis of adipocytes required sortilin expression. (A) Immunoblot analysis of sortilin expression and apoptosis/necroptosis markers in adipocytes differentiated from C 3 H 10 T 1/2 cells. (B) Immunoblot analysis of sortilin in adipocytes differentiated from C 3 H 10 T 1/2 treated with si. RNA or scrambled sequence controls (negative controls) (mean &#x 000 B 1; SEM; n = 4, DOI: 10. 14336/AD. 2019. 0810 &#x 0002 A; p &#x 0003 C; 0. 001). (C) Immunoblot analysis of cell surface protein detection in adipocytes differentiated from C 3 H 10 T 1/2 cells treated with vehicle or pro-BDNF (10 ng/ml) for 24 h (n = 4, means &#x 000 B 1; SEM, &#x 0002 A; p &#x 0003 C; 0. 001). Full images of Western blots are shown in supplementary Fig. 5.