AgeRelated Disruption of SteadyState Thymic Medulla Provokes Autoimmune

Age-Related Disruption of Steady-State Thymic Medulla Provokes Autoimmune Phenotype via Perturbing Negative Selection Jiangyan Xia 1, 2, # ; Hongjun Wang 1, # ; Jianfei Guo 1, 3, # ; Zhijie Zhang 1 ; Brandon Coder 1 ; Dong-Ming Su 1 ; 1 Department of Molecular Biology and Immunology, University of North Texas Health Science Center at Fort Worth, TX, 76107, USA ; 2 Southeast University Affiliated Zhongda Hospital, Nanjing, China ; 3 Department of Radiology, University of Texas Southwestern Medical Center at Dallas, TX, 75390, USA ; Figure 1. Generation of two-dimensional thymic cysts primarily in the thymic medullary region by Fox. N 1 flox fx deletion through a low level spontaneous leakage of ubiquitous Cre. ER T. A Representative H&E staining of early middle-aged 9 -month-old WT left panel and fxfx-u. Cre. ER T middle and right panels thymi at × 4 and × 10 magnifications. B - D Representative immunofluorescence staining of K 8 green versus K 5 red or K 8 green versus Claudin-3, 4 red shows two-dimensional thymic cysts in cryosections of early middle-aged fxfx-u. Cre. ER T thymi. Panel B shows that the cysts are mostly localized in the medullary K 5 + region; Panel C shows that the cystic epithelial lining was Claudin-3, 4 positive; Panel D shows the two-dimensional thymic cysts located in the thymic medullary region at low magnification. This experiment was null, 3(3), 248 -259. Doi: null per group, with essentially identical results. M = medulla; C = cortex. The magnifications of the objective lens are indicated. done in 2– 3 biological replicates