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Age-related CCL 12 Aggravates Intracerebral Hemorrhage-induced Brain Injury via Recruitment of Macrophages and T Lymphocytes Huang Jiacheng, Yang Guoqiang, Xiong Xiaoyi, Wang Maolin, Yuan Junjie, Zhang Qin, Gong Changxiong, Qiu Zhongming, Meng Zhaoyou, Xu Rui, Chen Qiong, Chen Ru, Xie Lexing, Xie Qi, Zi Wenjie, Jiang Guohui, Zhou Yu, Yang Qingwu 1 Department of Neurology, Xinqiao Hospital, the Army Medical University (Third Military Medical University), Chongqing 400037, China. 1 Central Laboratory, Xinqiao Hospital, the Army Medical University (Third Military Medical University), Chongqing 400037, China. Figure 6. Peripheral CCL 12 aggravates the ICH-induced damage by recruiting peripheral macrophages and CD 3+ T lymphocytes. (A) Representative fluorescence microscopy images show infiltrating F 4/80+ cells (macrophages) in the perihematomal tissues (the arrows indicate F 4/80+ cells; blue = 4&#x 000 B 0; -6 -diamidino-2 -phenylindole &#x 0005 B; DAPI&#x 0005 D; and red = F 4/80). (B) Representative fluorescence microscopy images show infiltrating Aging and Disease, 2020, 11(5), 1103 -1115. DOI: 10. 14336/AD. 2019. 1229 CD 3+ cells (T lymphocytes) in the perihematomal tissues (the arrows indicate CD 3+ cells; blue = DAPI, and green = CD 3). (C) CD 45+ CD 11 b+ cells were defined as macrophages and neutrophils and further analyzed to distinguish the two types of cells. (D) The number of macrophages, CD 3+ T cells and neutrophils in WT, CCL 12 -/, CCL 12 overexpression, and vehicle groups after ICH. Scale bars, 50 &#x 003 BC; m. The bar graphs show the means &#x 000 B 1; SDs. P -values were determined by ANOVA. &#x 0002 A; P &#x 0003 C; 0. 05; &#x 0002 A; P &#x 0003 C; 0. 01; ns, not significant.