Advanced Light Source Advanced Light Source 8 2

Advanced Light Source 8. 2. 1 HHMI 8. 2. 2 8. 3. 1 Berkeley

ALS 8. 3. 1 Team Acknowledgments Beamline 8. 3. 1 staff Jane Tanamachi George

Increasing the structure determination success rate 1 Crystal triage technology a) Simulate the experiment

MAD phasing simulation Correlation coefficient to correct model mlphare results Anomalous signal to noise

$fraction unconverted Damage changes absorbance 1. 0 0. 0 Holton, J. M. (2007). J.$

Re-centering beamline microscope reference image

Interleaved Scheduling experiment queue Minor 30 s Choe 120 s Alberta 60 s Choe

You don’t have to use Fluidigm chips… … but you will probably want to.

Laue spot from lysozyme 10 s exposure after 30 s

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Advanced Light Source

Advanced Light Source 8. 2. 1 HHMI 8. 2. 2 8. 3. 1 Berkeley Center for Structural Biology 5. 0. 2 5. 0. 1 UC Berkeley 5. 0. 3 Plexxikon MBC 4. 2. 2 ALS PX ca 2007 SBDR MAGGIE 12. 3. 1 SIBYLS IDAT

ALS 8. 3. 1 Team Acknowledgments Beamline 8. 3. 1 staff Jane Tanamachi George Meigs

Increasing the structure determination success rate 1 Crystal triage technology a) Simulate the experiment to discover minimum required data quality b) Understand radiation damage c) Combine these criteria into smarter strategy programs 2 Improve screening infrastructure a) offline targeting and unattended execution b) Room temperature tray screening

Is it real, or is it MLFSOM?

MAD phasing simulation Correlation coefficient to correct model mlphare results Anomalous signal to noise ratio

$fraction unconverted Damage changes absorbance 1. 0 0. 0 Holton, J. M. (2007). J.$

fraction unconverted Damage changes absorbance 1. 0 0. 0 Holton, J. M. (2007). J. Synchr. Rad. 14, 51 -72.

cool hand luke

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Re-centering beamline microscope reference image