ABRF 2005 Microarray Roundtable Microarray Platform Comparability Importance
ABRF 2005 Microarray Roundtable Microarray Platform Comparability: Importance of Informatics & Design Facilitator: Dr. Andrew I. Brooks University of Rochester Medical Center University of Medicine & Dentistry of New Jersey / Rutgers University
Roundtable Panelist l Academic l l Anna Rogojina, Ph. D. l St. Judes Mark Parrish, Ph. D. l Rosetta Inpharmatics Shawn Levy, Ph. D. l Vanderbilt Margaret Cam, Ph. D. l NIDDK, NIH l Industrial l l Peter Webb, Ph. D. l Agilent Technologies Steven Smith l Nimblegen Systems Timothy Sendera, Ph. D. l GE Healthcare Stephen Lincoln l Affymetrix John Burrill, Ph. D. l Applied Biosystems
Roundtable Rules l l A series of pre-decided questions will be asked by our Academic panelists. All Industrial panelists will be given an opportunity to answer. Questions will be taken by the audience a the end of the program (please state your name and institution). NO marketing, product promotion or derogatory targeted questions or comments will be allowed by the facilitator. *Biographies, Panel Questions and Literature pertaining to roundtable objectives is available in the back of the room and will be posted to the MARG website *Transcripts from the roundtable will be made available on the MARG website
Technology Overview I l GE Healthcare l l l Genetic content from public domain 30 mer oligonucleotides (spotted) In “most” cases one probe per gene Fluorescent detection One Color Affymetrix l l l Genetic content from public domain 25 mer oligonucelotides (in situ synthesis) Multiple probe sets per gene (3’UTR) Fluorescent detection One Color
Technology Overview II l Agilent Technologies l l l Catalogue array content from public domain 60 mer oligonucleotides (in situ synthesis) In “most” cases one probe per gene Fluorescent detection Two-Color Nimblegen Systems l l l Catalogue array content from public domain Variety of oligo lengths (24 -85 bp) (in situ synthesis) Number of probes selected by investigator Fluorescent detection One or Two Color
Technology Overview III l Applied Biosystems l l l Genetic content from Celera Discovery System 60 mer oligonucleotides (spotted) 85% single probe per gene Chemiluminescence detection One Color
- Slides: 6