A York Perspective Imaging at York Peter OToole
- Slides: 21
A York Perspective Imaging at York Peter O’Toole pot 1@york. ac. uk @York. Bioimaging I&C Team Meg Stark (EM), Karen Hogg (FACS, LSCM), Karen Hodgkinson (EM, FACS, LSCM), Graeme Park (LSCM, Light Sheet), Jo Marrison (MP-LSCM, Light Sheet), ? ? ? (LM/EM/Flow) Ian Morrison (e. SRM/FEM), Laura Wiggins (AI), Rakesh Suman (Ptychography), Adam Dickinson (Ptychography)
Department of Biology Introducing the Department of Biology and Bioscience Technology Facility Imaging & Cytometry Cancer Research Infection and Immunity Microbiology Plant BIology Genomics Proteomics Bioscience Technology Facility Protein Production Molecular Interactions Cell and developmental biology Biochemistry and biophysics Molecular and cellular medicine Bioinformatics and mathematical biology Bio. Informatics
Who we work with … Customers and clients The Bioscience Technology Facility provides services to: York academics (Biology, Chemistry, Hull York Medical School, Archaeology, Environment, Physics, Electronics) External Academics Commercial companies: From SME to large Pharma With a proven track record and over 50 different clients per year.
University of York / Maastricht University strategic alliance Imaging Focus
Department of Biology Introducing the Department of Biology and Bioscience Technology Facility Imaging & Cytometry Cancer Research Infection and Immunity Microbiology Plant BIology Genomics Proteomics Bioscience Technology Facility Protein Production Molecular Interactions Cell and developmental biology Biochemistry and biophysics Molecular and cellular medicine Bioinformatics and mathematical biology Bio. Informatics
Why Microscopy? Genomic Data Proteomic Data Biochemical Analysis Microscopy ……. and Cytometry
Current and future developments Imaging Technology Stem Cell Pathways Cancer Cell Control Immunological Effects Biochemical Mechanisms Biophysical Interactions Biophysical Dynamics Higher Sensitivity Higher Resolution Higher Multiplex Higher Throughput Greater Quantification Live Cell/in vivo Correlated Microscopy Areas of development Science up and down scale Analysis, analysis! (modelling, bridging technologies, increasing complexity)– Correlating to other ‘omics
About Us Central Facility and Research Lab Areas of Focus International Hub – East African Disease Beta-test and International Reference Site New Technologies Label-Free, Live-Cell (Ptychography) Ultra-High Tissue Phenotyping Higher resolution (e. SRM) and CLEM Ultrafast Super Resolution Microscopy Raman Microscopy
International Hub Developing capacity for flow cytometry / histopathology in East Africa Region This project is part of the EDCTP 2 Programme supported by the European Union (RIA 2016 V-1640)
New Technology A molecular pathology pipeline to study disease mechanisms Dx Flow/WB RNA-Seq Parasite NGS Nanostring / DSP biopsy IHC / RNA FISH Epidermis Signature Dermis MS imaging NLRP 3 CD 68 Mouse 1 -n Canid 1 -n Signature www. leishpathnet. org Data sharing / integration Patient 1 -n
Beta-Test and Reference Site Current collaborations
Ptychography Label-Free Imaging Minimal perturbations Quantitative Prolonged Live-Cell Imaging
B 104 Neuronal Cells - Brightfield 50µm
B 104 Neuronal Cells - Brightfield 50µm
The Individual v The Population
Dyson An example of British engineering Elegant and yet simple Efficient and quiet A world leader …but still a vacuum cleaner
Why? What makes the difference? Is she bigger? Is she skinnier? Does she pack more protein? Use ptychography to measure these aspects…
Other Measurements: Images to Metrics • • • Count Area Perimeter Length/width Circularity Quantitative Phase Data • • Mass Volume Thickness Surface profile/texture Motility/temporal Data • • • Speed Displacement Euclidean Distance Meandering index Instantaneous velocity Morphological Data etc…
Applications in Health Sciences Neurobiology Immunology Cancer Research Stem Cell Developmental Biology Microbiology Cancer Research Infection and Immunity Microbiology Plant BIology Cell and developmental biology Biochemistry and biophysics Molecular and cellular medicine Bioinformatics and mathematical biology
Why Microscopy? Genomic Data Proteomic Data Biochemical Analysis Microscopy
Correlative Microscopy Genomic Data Proteo/Metabolomic Data Biochemical Data Correlative Techniques: Microscope to ‘omics To really understand the cell, the individual and, the population
Laurence otoole
Frc driver station mac
Silo perspective vs business process perspective
One point perspective staircase
Peter o toole york
Peter berger sociological perspective
Live cell imaging applications
Digital color imaging
Tissue doppler imaging
Ucsf nikon imaging center
Picture perfect imaging
Right arm medical term
Fundamentals of digital imaging
Chapter 39 digital imaging film and radiographs
Digital imagery definition
Imaging lifecycle services
Medical imaging workstations
Brain imaging
Diagnostic imaging
Velocity map imaging
Cytobot
Hardware agnostic imaging
